Identification of Ctenocephalides felis fleas as a host of Rickettsia felis, the agent of a spotted fever rickettsiosis in Yucatań, Mexico.

J. E. Zavala-Velázquez, J. E. Zavala-Castro, I. Vado-Solís, J. A. Ruiz-Sosa, C. G. Moron, Donald Bouyer, David Walker

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

In search for the vector of the recently recognized spotted fever rickettsiosis of the Yucatán, ticks, fleas, and lice were collected from vegetation and dogs in localities where seropositive persons had been found. The arthropods were examined by polymerase chain reaction (PCR) using primers for the genus-specific 17-kDa protein gene followed by restriction fragment length polymorphism (RFLP) and DNA sequencing. Eleven (20%) of 54 pools of Ctenocephalides felis fleas contained DNA of Rickettsia felis. None of 219 Amblyomma cajennense, 474 Rhiphicephalus sanguineus, 258 Boophilus sp. ticks, and 33 Poliplax species lice contained DNA of Rickettsia. The identity of the rickettsial DNA was confirmed as R. felis by PCR/RFLP for the citrate synthase and outer membrane protein A genes and by DNA sequencing. The results indicate that the host of R. felis in Yucatán is C. felis and suggest that the spotted fever rickettsiosis that has infected >5% of the population of the Yucatán and can present as a dengue-like illness is likely to be caused by R. felis.

Original languageEnglish (US)
Pages (from-to)69-75
Number of pages7
JournalVector Borne Zoonotic Dis
Volume2
Issue number2
StatePublished - Jun 2002
Externally publishedYes

Fingerprint

Rickettsia felis
Ctenocephalides
Siphonaptera
Mexico
Fever
Phthiraptera
Ticks
DNA Sequence Analysis
Restriction Fragment Length Polymorphisms
DNA
vif Genes
Rickettsia
Citrate (si)-Synthase
Polymerase Chain Reaction
Dengue
Arthropods
Membrane Proteins
Dogs
Population
Proteins

ASJC Scopus subject areas

  • Infectious Diseases
  • Parasitology

Cite this

Identification of Ctenocephalides felis fleas as a host of Rickettsia felis, the agent of a spotted fever rickettsiosis in Yucatań, Mexico. / Zavala-Velázquez, J. E.; Zavala-Castro, J. E.; Vado-Solís, I.; Ruiz-Sosa, J. A.; Moron, C. G.; Bouyer, Donald; Walker, David.

In: Vector Borne Zoonotic Dis, Vol. 2, No. 2, 06.2002, p. 69-75.

Research output: Contribution to journalArticle

Zavala-Velázquez, J. E. ; Zavala-Castro, J. E. ; Vado-Solís, I. ; Ruiz-Sosa, J. A. ; Moron, C. G. ; Bouyer, Donald ; Walker, David. / Identification of Ctenocephalides felis fleas as a host of Rickettsia felis, the agent of a spotted fever rickettsiosis in Yucatań, Mexico. In: Vector Borne Zoonotic Dis. 2002 ; Vol. 2, No. 2. pp. 69-75.
@article{384dcab9574a4dcdb04498c7134c9ff7,
title = "Identification of Ctenocephalides felis fleas as a host of Rickettsia felis, the agent of a spotted fever rickettsiosis in Yucatań, Mexico.",
abstract = "In search for the vector of the recently recognized spotted fever rickettsiosis of the Yucat{\'a}n, ticks, fleas, and lice were collected from vegetation and dogs in localities where seropositive persons had been found. The arthropods were examined by polymerase chain reaction (PCR) using primers for the genus-specific 17-kDa protein gene followed by restriction fragment length polymorphism (RFLP) and DNA sequencing. Eleven (20{\%}) of 54 pools of Ctenocephalides felis fleas contained DNA of Rickettsia felis. None of 219 Amblyomma cajennense, 474 Rhiphicephalus sanguineus, 258 Boophilus sp. ticks, and 33 Poliplax species lice contained DNA of Rickettsia. The identity of the rickettsial DNA was confirmed as R. felis by PCR/RFLP for the citrate synthase and outer membrane protein A genes and by DNA sequencing. The results indicate that the host of R. felis in Yucat{\'a}n is C. felis and suggest that the spotted fever rickettsiosis that has infected >5{\%} of the population of the Yucat{\'a}n and can present as a dengue-like illness is likely to be caused by R. felis.",
author = "Zavala-Vel{\'a}zquez, {J. E.} and Zavala-Castro, {J. E.} and I. Vado-Sol{\'i}s and Ruiz-Sosa, {J. A.} and Moron, {C. G.} and Donald Bouyer and David Walker",
year = "2002",
month = "6",
language = "English (US)",
volume = "2",
pages = "69--75",
journal = "Vector-Borne and Zoonotic Diseases",
issn = "1530-3667",
publisher = "Mary Ann Liebert Inc.",
number = "2",

}

TY - JOUR

T1 - Identification of Ctenocephalides felis fleas as a host of Rickettsia felis, the agent of a spotted fever rickettsiosis in Yucatań, Mexico.

AU - Zavala-Velázquez, J. E.

AU - Zavala-Castro, J. E.

AU - Vado-Solís, I.

AU - Ruiz-Sosa, J. A.

AU - Moron, C. G.

AU - Bouyer, Donald

AU - Walker, David

PY - 2002/6

Y1 - 2002/6

N2 - In search for the vector of the recently recognized spotted fever rickettsiosis of the Yucatán, ticks, fleas, and lice were collected from vegetation and dogs in localities where seropositive persons had been found. The arthropods were examined by polymerase chain reaction (PCR) using primers for the genus-specific 17-kDa protein gene followed by restriction fragment length polymorphism (RFLP) and DNA sequencing. Eleven (20%) of 54 pools of Ctenocephalides felis fleas contained DNA of Rickettsia felis. None of 219 Amblyomma cajennense, 474 Rhiphicephalus sanguineus, 258 Boophilus sp. ticks, and 33 Poliplax species lice contained DNA of Rickettsia. The identity of the rickettsial DNA was confirmed as R. felis by PCR/RFLP for the citrate synthase and outer membrane protein A genes and by DNA sequencing. The results indicate that the host of R. felis in Yucatán is C. felis and suggest that the spotted fever rickettsiosis that has infected >5% of the population of the Yucatán and can present as a dengue-like illness is likely to be caused by R. felis.

AB - In search for the vector of the recently recognized spotted fever rickettsiosis of the Yucatán, ticks, fleas, and lice were collected from vegetation and dogs in localities where seropositive persons had been found. The arthropods were examined by polymerase chain reaction (PCR) using primers for the genus-specific 17-kDa protein gene followed by restriction fragment length polymorphism (RFLP) and DNA sequencing. Eleven (20%) of 54 pools of Ctenocephalides felis fleas contained DNA of Rickettsia felis. None of 219 Amblyomma cajennense, 474 Rhiphicephalus sanguineus, 258 Boophilus sp. ticks, and 33 Poliplax species lice contained DNA of Rickettsia. The identity of the rickettsial DNA was confirmed as R. felis by PCR/RFLP for the citrate synthase and outer membrane protein A genes and by DNA sequencing. The results indicate that the host of R. felis in Yucatán is C. felis and suggest that the spotted fever rickettsiosis that has infected >5% of the population of the Yucatán and can present as a dengue-like illness is likely to be caused by R. felis.

UR - http://www.scopus.com/inward/record.url?scp=0036627704&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036627704&partnerID=8YFLogxK

M3 - Article

VL - 2

SP - 69

EP - 75

JO - Vector-Borne and Zoonotic Diseases

JF - Vector-Borne and Zoonotic Diseases

SN - 1530-3667

IS - 2

ER -