Identification of differently expressed genes involved in the regression and development of the chicken müllerian duct

Yonju Ha, Akira Tsukada, Noboru Saito, David Zadworny, Kiyoshi Shimada

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Müllerian ducts of male chickens undergo regression around day 12 of incubation, but the underlining mechanisms remain unclear. The purpose of this study was to identify factors that contribute to regression of the Müllerian duct in the chicken. We first employed annealing control primer-based RT-PCR to screen candidate genes differentially expressed in the Müllerian ducts between male and female. Four differentially expressed genes (MSX2, GAL10, VCP and PLCH1) were partially sequenced. The expression of mRNA of the latter genes and MSX1 in the male and female Müllerian ducts were compared at 7.5, 8 and 9 days of incubation using semi-quantitative RT-PCR. The results indicated that both MSX1 and MSX2 mRNA was highly expressed in the male Müllerian duct at day 9 of incubation, whereas, PLCH1 mRNA was lower in the male duct at day 9 of incubation compared to that of the female duct. Although VCP mRNA was expressed in both left and right female Müllerian ducts, no expression was detected in the male duct. Whole mount in situ hybridyzation analysis showed that the expression of MSX1 and MSX2 mRNA were localized specifically in the mesenchymal cells of the male Müllerian duct at day 9 of incubation. In contrast, VCP mRNA expression was observed in both mesenchymal and epithelial cells of the female Müllerian duct but not detected in the male duct. These results suggest that both up-regulation of MSX1 and MSX2 mRNA expression is involved in the regression of the Müllerian duct in male chicken embryo, whereas VCP expression is involved in development of the female duct.

Original languageEnglish (US)
Pages (from-to)1135-1141
Number of pages7
JournalInternational Journal of Developmental Biology
Volume52
Issue number8
DOIs
StatePublished - 2008
Externally publishedYes

Fingerprint

Chickens
Messenger RNA
Genes
Polymerase Chain Reaction
Up-Regulation
Embryonic Structures
Epithelial Cells

Keywords

  • Annealing control primer-based RT-PCR
  • Chicken
  • Embryo
  • Müllerian duct

ASJC Scopus subject areas

  • Developmental Biology
  • Embryology

Cite this

Identification of differently expressed genes involved in the regression and development of the chicken müllerian duct. / Ha, Yonju; Tsukada, Akira; Saito, Noboru; Zadworny, David; Shimada, Kiyoshi.

In: International Journal of Developmental Biology, Vol. 52, No. 8, 2008, p. 1135-1141.

Research output: Contribution to journalArticle

Ha, Yonju ; Tsukada, Akira ; Saito, Noboru ; Zadworny, David ; Shimada, Kiyoshi. / Identification of differently expressed genes involved in the regression and development of the chicken müllerian duct. In: International Journal of Developmental Biology. 2008 ; Vol. 52, No. 8. pp. 1135-1141.
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abstract = "M{\"u}llerian ducts of male chickens undergo regression around day 12 of incubation, but the underlining mechanisms remain unclear. The purpose of this study was to identify factors that contribute to regression of the M{\"u}llerian duct in the chicken. We first employed annealing control primer-based RT-PCR to screen candidate genes differentially expressed in the M{\"u}llerian ducts between male and female. Four differentially expressed genes (MSX2, GAL10, VCP and PLCH1) were partially sequenced. The expression of mRNA of the latter genes and MSX1 in the male and female M{\"u}llerian ducts were compared at 7.5, 8 and 9 days of incubation using semi-quantitative RT-PCR. The results indicated that both MSX1 and MSX2 mRNA was highly expressed in the male M{\"u}llerian duct at day 9 of incubation, whereas, PLCH1 mRNA was lower in the male duct at day 9 of incubation compared to that of the female duct. Although VCP mRNA was expressed in both left and right female M{\"u}llerian ducts, no expression was detected in the male duct. Whole mount in situ hybridyzation analysis showed that the expression of MSX1 and MSX2 mRNA were localized specifically in the mesenchymal cells of the male M{\"u}llerian duct at day 9 of incubation. In contrast, VCP mRNA expression was observed in both mesenchymal and epithelial cells of the female M{\"u}llerian duct but not detected in the male duct. These results suggest that both up-regulation of MSX1 and MSX2 mRNA expression is involved in the regression of the M{\"u}llerian duct in male chicken embryo, whereas VCP expression is involved in development of the female duct.",
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