Identification of epitopes on the E glycoprotein of Saint Louis encephalitis virus using monoclonal antibodies

J. T. Roehrig, J. H. Mathews, D. W. Trent

Research output: Contribution to journalArticle

113 Scopus citations

Abstract

Twenty-one hybridomas producing monoclonal antibodies specific for the E glycoprotein of St. Louis encephalitis (SLE) virus, strain MSI-7, have been isolated. Serologic reactivities were initially determined by cross-reactivity indirect immunofluorescence assays using 22 strains of SLE virus and 8 other related flaviviruses. Four groups demonstrating type-, subcomplex-, supercomplex-, and group-specific reactivity patterns were identified. Analysis of hemagglutination-inhibition (HI) and virus neutralization (N) subdivided the cross-reactivity groups into eight epitopes (E-1a,b,c,d, E-2, E-3, and E-4a,b). The antibodies could detect strain differences between SLE viruses isolated from various geographic areas. Analysis of the spatial arrangements of these epitopes using competitive binding assays with representative antibodies possessing similar binding avidities, indicated that the protein was a continuum of six overlapping domains.

Original languageEnglish (US)
Pages (from-to)118-126
Number of pages9
JournalVirology
Volume128
Issue number1
DOIs
StatePublished - Jul 15 1983

ASJC Scopus subject areas

  • Virology

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