Identification of experimentally induced colitis by in vitro nuclear magnetic resonance.

R. H. Rolandelli, S. H. Saul, R. G. Settle, D. O. Jacobs, R. L. Singer, G. L. Wolf, J. L. Rombeau

Research output: Contribution to journalArticlepeer-review

4 Scopus citations


The present study determined whether in vitro nuclear magnetic resonance could be used to assess experimentally induced colitis in rats. Acute colitis was induced in 6 Sprague-Dawley rats by acetic acid enema, while 6 control animals received saline enemas. All animals were sacrificed 24 hours post-enema, and NMR relaxation times, T1 and T2, of colonic samples were determined on a 10 MHz spin analyzer (RADX, Houston, TX). Colonic water content was determined on the same samples by desiccation. Colitis animals showed significantly higher T1 and T2 relaxation times and tissue water content than controls. T1 and T2 times correlated significantly with tissue water content. Twelve additional animals were studied histologically, six of which received acetic acid enemas and showed extensive transmural colitis. Our results suggest that in vivo proton NMR might be a useful means of non-invasively assessing the degree of colonic inflammation.

Original languageEnglish (US)
Pages (from-to)197-203
Number of pages7
JournalPhysiological chemistry and physics and medical NMR
Issue number2
StatePublished - 1985
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Physiology
  • Spectroscopy


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