Identification of gene expression profiles in Leishmania major infection by integrated bioinformatics analyses

Özlem Ulusan, Ufuk Mert, Aygül Sadıqova, Sercan Öztürk, Ayse Caner

Research output: Contribution to journalArticle

Abstract

Gene expression profiling in mouse models of leishmaniasis has given useful information to understand the molecular pathways active in lesions and to discover new diagnostic/therapeutic targets. Although the host response plays a critical role in protection from leishmaniasis and promoting disease severity, there are still unexplained aspects in the mechanism of non-healing cutaneous lesions, which need biomarkers for both targeted- therapy and diagnosis. To address this, transcriptional profiling of the skin lesions obtained from BALB/c mice infected with Leishmania major and healthy skin from naïve mice were evaluated by bioinformatics analysis, and then the results were validated by Revers Transcriptase-PCR. Five genes among the up-regulated differentially expressed genes named FCGR4, CCL4, CXCL9, Arg1 and IL-1β were found to have relatively high diagnostic value for CL due to L. major. Pathway analysis revealed that Triggering Receptor Expressed On Myeloid Cells 1 (TREM1) signaling pathways are active in cutaneous lesions, providing new insights for the understanding and treatment of leishmaniasis.

Original languageEnglish (US)
Article number105517
JournalActa Tropica
Volume208
DOIs
StatePublished - Aug 2020

Keywords

  • BALB/c mice
  • Gene expression
  • Leishmania major (L. major)
  • Macrophages
  • TREM signaling

ASJC Scopus subject areas

  • Parasitology
  • veterinary (miscalleneous)
  • Insect Science
  • Infectious Diseases

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