Identification of genes leading to glucocorticoid-induced leukemic cell death

E. B. Thompson, M. S. Webb, A. L. Miller, Y. Fofanov, B. H. Johnson

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

Glucocorticoidal steroids (GC) are capable of causing apoptotic death of many varieties of lymphoid cells; consequently, GC are used in therapy for many lymphoid malignancies. Gene transcription in the GC-treated cells is required for subsequent apoptosis, but only a few of the actual genes involved have been identified. We employed gene microarray analysis to find the network of genes involved in GC-evoked cell death, using three clones derived from the CEM lymphoid leukemia cell line. Clone C1-15 was resistant to GC-evoked apoptosis, although not necessarily to GC-induced gene transcription; the other two underwent apoptosis in the presence of GC. Clone C7-14 was subcloned from the apoptosis-sensitive parental C7 clone to establish karyotypic uniformity. The second sensitive clone, C1-6, was a spontaneous revertant from parental resistant clone C1. A period of ≥24 h in the constant presence of receptor-occupying concentrations of synthetic GC dexamethasone (Dex) was necessary for apoptosis to begin. To identify the steps leading to this dramatic event, we identified the changes in gene expression in the 20-h period preceding the onset of overt apoptosis. Cells in the log phase of growth were treated with 10-6 M Dex, and 2-20 h later, mRNA was prepared and analyzed using the Affymetrix HG_U95Av2 chip, containing probes for about 12,600 genes. Of these, approximately 6,000 were expressed above background. Comparisons of the basal and expressed genes in the three clones led to several conclusions: The Dex-sensitive clones shared the regulation of a limited set of genes. The apoptosis-resistant clone C1-15 showed Dex effects on a largely different set of genes. Promoter analysis of the regulated genes suggested that primary gene targets for GC often lack a classic GC response element.

Original languageEnglish (US)
Pages (from-to)821-825
Number of pages5
JournalLipids
Volume39
Issue number8
StatePublished - Aug 1 2004

ASJC Scopus subject areas

  • Biochemistry
  • Organic Chemistry
  • Cell Biology

Fingerprint Dive into the research topics of 'Identification of genes leading to glucocorticoid-induced leukemic cell death'. Together they form a unique fingerprint.

  • Cite this

    Thompson, E. B., Webb, M. S., Miller, A. L., Fofanov, Y., & Johnson, B. H. (2004). Identification of genes leading to glucocorticoid-induced leukemic cell death. Lipids, 39(8), 821-825.