Identification of L-type calcium channels associated with kappa opioid receptors in human placenta

Bojana Cemerikic, Rahayla Zamah, Mahmoud Ahmed

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Transduction pathways of kappa receptor activation are not fully understood. Human placenta at term expresses only this type of opioid receptors and therefore offers a unique advantage for such investigations. It has previously been postulated that kappa receptors-mediated modulation of acetylcholine and placental lactogen release from human placentas require the influx of extracellular calcium and into the cells, possibly via voltage- dependent channels. We report here that another opioid-regulated placental function, the release of human chorionic gonadotropin (hCG), depends on extracellular calcium and the modality of its influx via L-type channels. Data presented demonstrated that the stimulation of hCG secretion by the kappa-selective agonist U69,593 was abolished in presence of either EGTA or the calcium channel blocker nifedipine. Results obtained on the combined effect of opioids and dihydropyridines indicated that placental kappa opioid receptors could be directly coupled to L-type calcium channels. The identification of the latter in villus membrane preparations, reported here for the first time, further contributes to the hypothesis that, in human placenta, kappa receptors-linked transduction mechanisms involve calcium and its conductance across villus membranes.

Original languageEnglish (US)
Pages (from-to)261-272
Number of pages12
JournalJournal of Molecular Neuroscience
Volume10
Issue number3
StatePublished - 1998
Externally publishedYes

Fingerprint

L-Type Calcium Channels
kappa Opioid Receptor
Placenta
Chorionic Gonadotropin
Calcium
Opioid Analgesics
Dihydropyridines
Membranes
Placental Lactogen
Egtazic Acid
Calcium Channel Blockers
Opioid Receptors
Nifedipine
Acetylcholine
Chemical activation
Modulation
Electric potential

Keywords

  • Bay K 8644
  • Calcium channels
  • Chorionic gonadotropin
  • EGTA
  • Human placenta
  • Kappa receptors
  • Nifedipine
  • Nor-binaltorphimine
  • U69,593

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry
  • Genetics

Cite this

Identification of L-type calcium channels associated with kappa opioid receptors in human placenta. / Cemerikic, Bojana; Zamah, Rahayla; Ahmed, Mahmoud.

In: Journal of Molecular Neuroscience, Vol. 10, No. 3, 1998, p. 261-272.

Research output: Contribution to journalArticle

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