Identification of metastasis-associated genes in early stage non-small cell lung cancer by subtractive hybridization

Wen Bing Wang, Sebastian Böing, Xiao Qin Zhou, Ping Ji, Yin Dong, Qin Yao, Carsten Müller-Tidow

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Non-small cell lung cancer (NSCLC) is a leading cause of death and a substantial fraction of patients with surgically resected disease ultimately dies due to distant metastasis. To identify gene expression differences in early stage adenocarcinoma that either did or did not metastasize within a 5-year period, we employed a subtractive hybridization strategy of pooled RNA from primary adenocarcinomas (stage I) of the lung. Individual clones (n = 225) of the subtracted cDNA library were sequenced. Further analyses of mRNA expression levels in a cohort of 70 NSCLC patients (stage I to IIIA) showed that the metastasis association of the identified genes was stage and histology specific. Cox regression analyses identified two genes (EIF4A1, MALA1) to be independent prognostic parameters for patients' survival in stage I and II disease. These findings could help to identify early-stage NSCLC patients at high risk for the development of distant metastasis.

Original languageEnglish (US)
Pages (from-to)273-278
Number of pages6
JournalActa Biochimica et Biophysica Sinica
Volume34
Issue number3
StatePublished - 2002
Externally publishedYes

Fingerprint

Non-Small Cell Lung Carcinoma
Genes
Cells
Neoplasm Metastasis
Histology
Gene Library
Gene expression
Adenocarcinoma
RNA
Messenger RNA
Cause of Death
Clone Cells
Regression Analysis
Gene Expression
Lung
Survival
Subtractive Hybridization Techniques

Keywords

  • Expression
  • Gene
  • Metastasis
  • Non-small cell lung cancer
  • Subtractive hybridization

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics

Cite this

Identification of metastasis-associated genes in early stage non-small cell lung cancer by subtractive hybridization. / Wang, Wen Bing; Böing, Sebastian; Zhou, Xiao Qin; Ji, Ping; Dong, Yin; Yao, Qin; Müller-Tidow, Carsten.

In: Acta Biochimica et Biophysica Sinica, Vol. 34, No. 3, 2002, p. 273-278.

Research output: Contribution to journalArticle

Wang, WB, Böing, S, Zhou, XQ, Ji, P, Dong, Y, Yao, Q & Müller-Tidow, C 2002, 'Identification of metastasis-associated genes in early stage non-small cell lung cancer by subtractive hybridization', Acta Biochimica et Biophysica Sinica, vol. 34, no. 3, pp. 273-278.
Wang, Wen Bing ; Böing, Sebastian ; Zhou, Xiao Qin ; Ji, Ping ; Dong, Yin ; Yao, Qin ; Müller-Tidow, Carsten. / Identification of metastasis-associated genes in early stage non-small cell lung cancer by subtractive hybridization. In: Acta Biochimica et Biophysica Sinica. 2002 ; Vol. 34, No. 3. pp. 273-278.
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