Identification of Novel Viruses Using VirusHunter -- an Automated Data Analysis Pipeline

Guoyan Zhao, Siddharth Krishnamurthy, Zhengqiu Cai, Vsevolod L. Popov, Amelia P. Travassos da Rosa, Hilda Guzman, Song Cao, Herbert W. Virgin, Robert B. Tesh, David Wang

Research output: Contribution to journalArticle

50 Scopus citations

Abstract

Quick and accurate identification of microbial pathogens is essential for both diagnosis and response to emerging infectious diseases. The advent of next-generation sequencing technology offers an unprecedented platform for rapid sequencing-based identification of novel viruses. We have developed a customized bioinformatics data analysis pipeline, VirusHunter, for the analysis of Roche/454 and other long read Next generation sequencing platform data. To illustrate the utility of VirusHunter, we performed Roche/454 GS FLX titanium sequencing on two unclassified virus isolates from the World Reference Center for Emerging Viruses and Arboviruses (WRCEVA). VirusHunter identified sequences derived from a novel bunyavirus and a novel reovirus in the two samples respectively. Further sequence analysis demonstrated that the viruses were novel members of the Phlebovirus and Orbivirus genera. Both Phlebovirus and Orbivirus genera include many economic important viruses or serious human pathogens.

Original languageEnglish (US)
Article numbere78470
JournalPloS one
Volume8
Issue number10
DOIs
StatePublished - Oct 22 2013

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

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    Zhao, G., Krishnamurthy, S., Cai, Z., Popov, V. L., Travassos da Rosa, A. P., Guzman, H., Cao, S., Virgin, H. W., Tesh, R. B., & Wang, D. (2013). Identification of Novel Viruses Using VirusHunter -- an Automated Data Analysis Pipeline. PloS one, 8(10), [e78470]. https://doi.org/10.1371/journal.pone.0078470