Identification of protective components of two major outer membrane proteins of spotted fever group rickettsiae

C. M. Díaz-Montero, H. M. Feng, P. A. Crocquet-Valdes, David Walker

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Fragments representing the genes of the two major outer membrane proteins of spotted fever group rickettsiae (rOmpA and rOmpB) were tested as DNA vaccines. Immunizations with each of three fragments (rompA4999-6710, rompB1550-2738, and rompB2459-4123) conferred a degree of protection on vaccinated mice against virulent rickettsial challenge. Protection was achieved when DNA immunizations were followed by booster immunizations with the homologous recombinant protein. Proliferation and gamma-interferon secretion were detected after in vitro stimulation of lymphocytes from immunized animals with whole Rickettsia conorii antigen. The data validate particular segments of rOmpA and rOmpB as potent immunogens and hence as sources of immunostimulatory elements with specificity for T lymphocytes, which are the key effectors of protective immunity against rickettsial infections.

Original languageEnglish (US)
Pages (from-to)371-378
Number of pages8
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume65
Issue number4
StatePublished - 2001

Fingerprint

Rickettsia
Immunization
Membrane Proteins
Fever
Rickettsia conorii
Secondary Immunization
DNA Vaccines
Lymphocyte Activation
Recombinant Proteins
Interferon-gamma
Immunity
T-Lymphocytes
Antigens
DNA
Infection
Genes
In Vitro Techniques
Rickettsia rOmpA

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

Cite this

Identification of protective components of two major outer membrane proteins of spotted fever group rickettsiae. / Díaz-Montero, C. M.; Feng, H. M.; Crocquet-Valdes, P. A.; Walker, David.

In: American Journal of Tropical Medicine and Hygiene, Vol. 65, No. 4, 2001, p. 371-378.

Research output: Contribution to journalArticle

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