Identification of proteins from Escherichia coli using two-dimensional semi-preparative electrophoresis and mass spectrometry

Elisabet Gustafsson, Kajsa Thorén, Thomas Larsson, Pia Davidsson, Karl Anders Karlsson, Carol L. Nilsson

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Escherichia coli is a gram-negative bacterium that causes sepsis and infections of the nervous system, and the digestive and urinary tracts. The availability of the complete nucleotide sequence encoding the E. coli K-12 genome has made this organism an excellent model for proteomic studies. Semi-preparative two-dimensional electrophoresis, including liquid phase isoelectric focusing (IEF), one-dimensional sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis (PAGE) and gel elution, have for the first time been used in combination with matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOFMS), electrospray tandem mass spectrometry and database searching for rapid separation of proteins from a uropathogenic strain of E. coli. The identity of 30 proteins, including the membrane protein nmpC, was obtained using this approach.

Original languageEnglish (US)
Pages (from-to)428-432
Number of pages5
JournalRapid Communications in Mass Spectrometry
Volume15
Issue number6
DOIs
StatePublished - 2001
Externally publishedYes

Fingerprint

Escherichia coli Proteins
Electrophoresis
Escherichia coli
Mass spectrometry
Neurology
Sodium Dodecyl Sulfate
Ionization
Desorption
Bacteria
Membrane Proteins
Proteins
Nucleotides
Genes
Gels
Availability
Lasers
Liquids

ASJC Scopus subject areas

  • Analytical Chemistry
  • Spectroscopy

Cite this

Identification of proteins from Escherichia coli using two-dimensional semi-preparative electrophoresis and mass spectrometry. / Gustafsson, Elisabet; Thorén, Kajsa; Larsson, Thomas; Davidsson, Pia; Karlsson, Karl Anders; Nilsson, Carol L.

In: Rapid Communications in Mass Spectrometry, Vol. 15, No. 6, 2001, p. 428-432.

Research output: Contribution to journalArticle

Gustafsson, Elisabet ; Thorén, Kajsa ; Larsson, Thomas ; Davidsson, Pia ; Karlsson, Karl Anders ; Nilsson, Carol L. / Identification of proteins from Escherichia coli using two-dimensional semi-preparative electrophoresis and mass spectrometry. In: Rapid Communications in Mass Spectrometry. 2001 ; Vol. 15, No. 6. pp. 428-432.
@article{10867eb378fa496abe65861071de4a19,
title = "Identification of proteins from Escherichia coli using two-dimensional semi-preparative electrophoresis and mass spectrometry",
abstract = "Escherichia coli is a gram-negative bacterium that causes sepsis and infections of the nervous system, and the digestive and urinary tracts. The availability of the complete nucleotide sequence encoding the E. coli K-12 genome has made this organism an excellent model for proteomic studies. Semi-preparative two-dimensional electrophoresis, including liquid phase isoelectric focusing (IEF), one-dimensional sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis (PAGE) and gel elution, have for the first time been used in combination with matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOFMS), electrospray tandem mass spectrometry and database searching for rapid separation of proteins from a uropathogenic strain of E. coli. The identity of 30 proteins, including the membrane protein nmpC, was obtained using this approach.",
author = "Elisabet Gustafsson and Kajsa Thor{\'e}n and Thomas Larsson and Pia Davidsson and Karlsson, {Karl Anders} and Nilsson, {Carol L.}",
year = "2001",
doi = "10.1002/rcm.243",
language = "English (US)",
volume = "15",
pages = "428--432",
journal = "Rapid Communications in Mass Spectrometry",
issn = "0951-4198",
publisher = "John Wiley and Sons Ltd",
number = "6",

}

TY - JOUR

T1 - Identification of proteins from Escherichia coli using two-dimensional semi-preparative electrophoresis and mass spectrometry

AU - Gustafsson, Elisabet

AU - Thorén, Kajsa

AU - Larsson, Thomas

AU - Davidsson, Pia

AU - Karlsson, Karl Anders

AU - Nilsson, Carol L.

PY - 2001

Y1 - 2001

N2 - Escherichia coli is a gram-negative bacterium that causes sepsis and infections of the nervous system, and the digestive and urinary tracts. The availability of the complete nucleotide sequence encoding the E. coli K-12 genome has made this organism an excellent model for proteomic studies. Semi-preparative two-dimensional electrophoresis, including liquid phase isoelectric focusing (IEF), one-dimensional sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis (PAGE) and gel elution, have for the first time been used in combination with matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOFMS), electrospray tandem mass spectrometry and database searching for rapid separation of proteins from a uropathogenic strain of E. coli. The identity of 30 proteins, including the membrane protein nmpC, was obtained using this approach.

AB - Escherichia coli is a gram-negative bacterium that causes sepsis and infections of the nervous system, and the digestive and urinary tracts. The availability of the complete nucleotide sequence encoding the E. coli K-12 genome has made this organism an excellent model for proteomic studies. Semi-preparative two-dimensional electrophoresis, including liquid phase isoelectric focusing (IEF), one-dimensional sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis (PAGE) and gel elution, have for the first time been used in combination with matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOFMS), electrospray tandem mass spectrometry and database searching for rapid separation of proteins from a uropathogenic strain of E. coli. The identity of 30 proteins, including the membrane protein nmpC, was obtained using this approach.

UR - http://www.scopus.com/inward/record.url?scp=0035063984&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035063984&partnerID=8YFLogxK

U2 - 10.1002/rcm.243

DO - 10.1002/rcm.243

M3 - Article

C2 - 11291121

AN - SCOPUS:0035063984

VL - 15

SP - 428

EP - 432

JO - Rapid Communications in Mass Spectrometry

JF - Rapid Communications in Mass Spectrometry

SN - 0951-4198

IS - 6

ER -