Identification of proteins from Escherichia coli using two-dimensional semi-preparative electrophoresis and mass spectrometry

Elisabet Gustafsson, Kajsa Thorén, Thomas Larsson, Pia Davidsson, Karl Anders Karlsson, Carol L. Nilsson

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

Escherichia coli is a gram-negative bacterium that causes sepsis and infections of the nervous system, and the digestive and urinary tracts. The availability of the complete nucleotide sequence encoding the E. coli K-12 genome has made this organism an excellent model for proteomic studies. Semi-preparative two-dimensional electrophoresis, including liquid phase isoelectric focusing (IEF), one-dimensional sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis (PAGE) and gel elution, have for the first time been used in combination with matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOFMS), electrospray tandem mass spectrometry and database searching for rapid separation of proteins from a uropathogenic strain of E. coli. The identity of 30 proteins, including the membrane protein nmpC, was obtained using this approach.

Original languageEnglish (US)
Pages (from-to)428-432
Number of pages5
JournalRapid Communications in Mass Spectrometry
Volume15
Issue number6
DOIs
StatePublished - Jan 1 2001

ASJC Scopus subject areas

  • Analytical Chemistry
  • Spectroscopy
  • Organic Chemistry

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