Identification of proteins to predict the molecular basis for the observed gender susceptibility in a rat model of alcoholic steatohepatitis by 2-D gel proteomics

Atrayee Banerjee, William Russell, Arul Jayaraman, Shashi K. Ramaiah

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Females are reported to be highly susceptible to alcoholic steatohepatitis (ASH) compared to the males. Although a variety of mechanisms have been proposed to explain this higher sensitivity of females, the precise mechanism is not well understood. The objective of this study was to identify changes in global protein expression in liver tissues of male and female rats with pathologically evident ASH by 2-DE (dimensional electrophoresis). ASH was induced in the SD (Sprague-Dawley) rats by feeding ethanol (EtOH) containing Lieber-DeCarli diet for 6 wk followed by a single injection of lipopolysaccharide (LPS, 10 mg/kg, i.p.). Higher liver injury in females in the ASH group as compared to the males was confirmed by HE stained liver sections. As identified by 2-DE, 22 protein-spots were differentially expressed in the females in the ASH group as compared to the males. Following identification of these proteins by MALDI-MS, they were mainly categorized into metabolism and oxidative stress-related proteins. The expression pattern of a few of these oxidative stress-related proteins like Ferritin Heavy chain (Ferritin-H chain), ER stress protein 60 (ER 60) and Heat-shock protein-60 (HSP 60) were verified by Western blotting. To conclude, the current study has identified a set of proteins that highlights potential novel mechanisms associated with higher liver injury noted in the female rat ASH model.

Original languageEnglish (US)
Pages (from-to)4327-4337
Number of pages11
JournalProteomics
Volume8
Issue number20
DOIs
StatePublished - Oct 2008
Externally publishedYes

Fingerprint

Alcoholic Fatty Liver
Proteomics
Rats
Gels
Liver
Heat-Shock Proteins
Proteins
Oxidative stress
Oxidative Stress
Apoferritins
Chaperonin 60
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Wounds and Injuries
Nutrition
Electrophoresis
Metabolism
Sprague Dawley Rats
Lipopolysaccharides
Ethanol
Western Blotting

Keywords

  • Alcohol
  • Female
  • Liver
  • Steatohepatitis

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry

Cite this

Identification of proteins to predict the molecular basis for the observed gender susceptibility in a rat model of alcoholic steatohepatitis by 2-D gel proteomics. / Banerjee, Atrayee; Russell, William; Jayaraman, Arul; Ramaiah, Shashi K.

In: Proteomics, Vol. 8, No. 20, 10.2008, p. 4327-4337.

Research output: Contribution to journalArticle

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