Identification of PVK/CAP2b neuropeptides from single neurohemal organs of the stable fly and horn fly via MALDI-TOF/TOF tandem mass spectrometry

Ronald J. Nachman, William Russell, Geoffrey M. Coast, David H. Russell, J. Allen Miller, Reinhard Predel

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

MALDI-TOF/TOF tandem mass spectrometry has been applied to determine the complete sequences of the PVK/CAP2b neuropeptides in the stable fly Stomoxys calcitrans and horn fly Haematobia irritans, insect pests of livestock. This peptidomic analysis of single neurohemal organ preparations allows the unambiguous assignment of internal Leu/Ile positions not distinguishable by previous mass spectrometric techniques. The sequences are as follows: Stoca-PVK/CAP2b-1, AGGASGLYAFPRVa; Stoca-PVK/CAP2b-2, NAKLYPVPRVa; and Haeir-PVK/CAP2b-1, AGGASGLYAFPRVa; Haeir-PVK/CAP2b-1, NAKLYPMPRVa. Both Stoca-PVK/CAP2b-1 and -2 stimulate Malpighian tubule fluid secretion in the stable fly, with EC50 values between 3 and 11 nM. The identification of these novel neuropeptides adds to our knowledge of the peptidomes of flies, and can aid in the development of neuropeptide-based control strategies of these insect pests.

Original languageEnglish (US)
Pages (from-to)521-526
Number of pages6
JournalPeptides
Volume27
Issue number3
DOIs
StatePublished - Mar 2006
Externally publishedYes

Fingerprint

Muscidae
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Tandem Mass Spectrometry
Neuropeptides
Mass spectrometry
Agriculture
Malpighian Tubules
Insect Control
Fluids and Secretions
Livestock
Fluids
Diptera
Insects
Circumventricular Organs

Keywords

  • CAP2b
  • Haematobia irritans
  • Insect
  • MALDI-TOF/TOF mass spectrometry
  • Neuropeptide
  • Peptidomics
  • Periviscerokinin
  • Stomoxys calcitrans

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Physiology
  • Cellular and Molecular Neuroscience

Cite this

Identification of PVK/CAP2b neuropeptides from single neurohemal organs of the stable fly and horn fly via MALDI-TOF/TOF tandem mass spectrometry. / Nachman, Ronald J.; Russell, William; Coast, Geoffrey M.; Russell, David H.; Miller, J. Allen; Predel, Reinhard.

In: Peptides, Vol. 27, No. 3, 03.2006, p. 521-526.

Research output: Contribution to journalArticle

Nachman, Ronald J. ; Russell, William ; Coast, Geoffrey M. ; Russell, David H. ; Miller, J. Allen ; Predel, Reinhard. / Identification of PVK/CAP2b neuropeptides from single neurohemal organs of the stable fly and horn fly via MALDI-TOF/TOF tandem mass spectrometry. In: Peptides. 2006 ; Vol. 27, No. 3. pp. 521-526.
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