Identification of PVK/CAP2b neuropeptides from single neurohemal organs of the stable fly and horn fly via MALDI-TOF/TOF tandem mass spectrometry

Ronald J. Nachman; William K. Russell; Geoffrey M. Coast; David H. Russell; J. Allen Miller; Reinhard Predel

doi:10.1016/j.peptides.2005.07.022

Identification of PVK/CAP2b neuropeptides from single neurohemal organs of the stable fly and horn fly via MALDI-TOF/TOF tandem mass spectrometry

Ronald J. Nachman
, William K. Russell
, Geoffrey M. Coast
, David H. Russell
, J. Allen Miller
, Reinhard Predel

Research output: Contribution to journal › Article › peer-review

20 Scopus citations

Abstract

MALDI-TOF/TOF tandem mass spectrometry has been applied to determine the complete sequences of the PVK/CAP2b neuropeptides in the stable fly Stomoxys calcitrans and horn fly Haematobia irritans, insect pests of livestock. This peptidomic analysis of single neurohemal organ preparations allows the unambiguous assignment of internal Leu/Ile positions not distinguishable by previous mass spectrometric techniques. The sequences are as follows: Stoca-PVK/CAP2b-1, AGGASGLYAFPRVa; Stoca-PVK/CAP2b-2, NAKLYPVPRVa; and Haeir-PVK/CAP2b-1, AGGASGLYAFPRVa; Haeir-PVK/CAP2b-1, NAKLYPMPRVa. Both Stoca-PVK/CAP2b-1 and -2 stimulate Malpighian tubule fluid secretion in the stable fly, with EC₅₀ values between 3 and 11 nM. The identification of these novel neuropeptides adds to our knowledge of the peptidomes of flies, and can aid in the development of neuropeptide-based control strategies of these insect pests.

Original language	English (US)
Pages (from-to)	521-526
Number of pages	6
Journal	Peptides
Volume	27
Issue number	3
DOIs	https://doi.org/10.1016/j.peptides.2005.07.022
State	Published - Mar 2006
Externally published	Yes

Keywords

CAP2b
Haematobia irritans
Insect
MALDI-TOF/TOF mass spectrometry
Neuropeptide
Peptidomics
Periviscerokinin
Stomoxys calcitrans

ASJC Scopus subject areas

Biochemistry
Physiology
Endocrinology
Cellular and Molecular Neuroscience

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10.1016/j.peptides.2005.07.022

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@article{e10ba6a102f8413aaeb293ee5152f824,

title = "Identification of PVK/CAP2b neuropeptides from single neurohemal organs of the stable fly and horn fly via MALDI-TOF/TOF tandem mass spectrometry",

abstract = "MALDI-TOF/TOF tandem mass spectrometry has been applied to determine the complete sequences of the PVK/CAP2b neuropeptides in the stable fly Stomoxys calcitrans and horn fly Haematobia irritans, insect pests of livestock. This peptidomic analysis of single neurohemal organ preparations allows the unambiguous assignment of internal Leu/Ile positions not distinguishable by previous mass spectrometric techniques. The sequences are as follows: Stoca-PVK/CAP2b-1, AGGASGLYAFPRVa; Stoca-PVK/CAP2b-2, NAKLYPVPRVa; and Haeir-PVK/CAP2b-1, AGGASGLYAFPRVa; Haeir-PVK/CAP2b-1, NAKLYPMPRVa. Both Stoca-PVK/CAP2b-1 and -2 stimulate Malpighian tubule fluid secretion in the stable fly, with EC50 values between 3 and 11 nM. The identification of these novel neuropeptides adds to our knowledge of the peptidomes of flies, and can aid in the development of neuropeptide-based control strategies of these insect pests.",

keywords = "CAP2b, Haematobia irritans, Insect, MALDI-TOF/TOF mass spectrometry, Neuropeptide, Peptidomics, Periviscerokinin, Stomoxys calcitrans",

author = "Nachman, \{Ronald J.\} and Russell, \{William K.\} and Coast, \{Geoffrey M.\} and Russell, \{David H.\} and Miller, \{J. Allen\} and Reinhard Predel",

note = "Funding Information: This study was supported by a Binational Agricultural Research and Development Grant (BARD \#IS-3356-02) (RJN), a grant from Deutsche Forschungsgemeinschaft (Predel 595/6-1), a Collaborative Research Grant (\#LST.CLG.979226) from the North Atlantic Treaty Organization (NATO) (RJN, GMC), a grant from the USDA/DOD DWFP Initiative (\#0500-32000-001-01R) (RJN), and grants provided to the Laboratory for Biological Mass Spectrometry at Texas A\&M University, College Station: R01 RR019587 NIH and DE-FG02-04ER15520 DOE. In addition, we acknowledge the capable technical assistance of Allison Strey, Pawel Zubrzak, and Nan Pryor of the Areawide Pest Management Research Unit, Southern Plains Agricultural Research Center.",

year = "2006",

month = mar,

doi = "10.1016/j.peptides.2005.07.022",

language = "English (US)",

volume = "27",

pages = "521--526",

journal = "Peptides",

issn = "0196-9781",

publisher = "Elsevier Inc.",

number = "3",

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TY - JOUR

T1 - Identification of PVK/CAP2b neuropeptides from single neurohemal organs of the stable fly and horn fly via MALDI-TOF/TOF tandem mass spectrometry

AU - Nachman, Ronald J.

AU - Russell, William K.

AU - Coast, Geoffrey M.

AU - Russell, David H.

AU - Miller, J. Allen

AU - Predel, Reinhard

N1 - Funding Information: This study was supported by a Binational Agricultural Research and Development Grant (BARD #IS-3356-02) (RJN), a grant from Deutsche Forschungsgemeinschaft (Predel 595/6-1), a Collaborative Research Grant (#LST.CLG.979226) from the North Atlantic Treaty Organization (NATO) (RJN, GMC), a grant from the USDA/DOD DWFP Initiative (#0500-32000-001-01R) (RJN), and grants provided to the Laboratory for Biological Mass Spectrometry at Texas A&M University, College Station: R01 RR019587 NIH and DE-FG02-04ER15520 DOE. In addition, we acknowledge the capable technical assistance of Allison Strey, Pawel Zubrzak, and Nan Pryor of the Areawide Pest Management Research Unit, Southern Plains Agricultural Research Center.

PY - 2006/3

Y1 - 2006/3

N2 - MALDI-TOF/TOF tandem mass spectrometry has been applied to determine the complete sequences of the PVK/CAP2b neuropeptides in the stable fly Stomoxys calcitrans and horn fly Haematobia irritans, insect pests of livestock. This peptidomic analysis of single neurohemal organ preparations allows the unambiguous assignment of internal Leu/Ile positions not distinguishable by previous mass spectrometric techniques. The sequences are as follows: Stoca-PVK/CAP2b-1, AGGASGLYAFPRVa; Stoca-PVK/CAP2b-2, NAKLYPVPRVa; and Haeir-PVK/CAP2b-1, AGGASGLYAFPRVa; Haeir-PVK/CAP2b-1, NAKLYPMPRVa. Both Stoca-PVK/CAP2b-1 and -2 stimulate Malpighian tubule fluid secretion in the stable fly, with EC50 values between 3 and 11 nM. The identification of these novel neuropeptides adds to our knowledge of the peptidomes of flies, and can aid in the development of neuropeptide-based control strategies of these insect pests.

AB - MALDI-TOF/TOF tandem mass spectrometry has been applied to determine the complete sequences of the PVK/CAP2b neuropeptides in the stable fly Stomoxys calcitrans and horn fly Haematobia irritans, insect pests of livestock. This peptidomic analysis of single neurohemal organ preparations allows the unambiguous assignment of internal Leu/Ile positions not distinguishable by previous mass spectrometric techniques. The sequences are as follows: Stoca-PVK/CAP2b-1, AGGASGLYAFPRVa; Stoca-PVK/CAP2b-2, NAKLYPVPRVa; and Haeir-PVK/CAP2b-1, AGGASGLYAFPRVa; Haeir-PVK/CAP2b-1, NAKLYPMPRVa. Both Stoca-PVK/CAP2b-1 and -2 stimulate Malpighian tubule fluid secretion in the stable fly, with EC50 values between 3 and 11 nM. The identification of these novel neuropeptides adds to our knowledge of the peptidomes of flies, and can aid in the development of neuropeptide-based control strategies of these insect pests.

KW - CAP2b

KW - Haematobia irritans

KW - Insect

KW - MALDI-TOF/TOF mass spectrometry

KW - Neuropeptide

KW - Peptidomics

KW - Periviscerokinin

KW - Stomoxys calcitrans

UR - https://www.scopus.com/pages/publications/33644666434

UR - https://www.scopus.com/pages/publications/33644666434#tab=citedBy

U2 - 10.1016/j.peptides.2005.07.022

DO - 10.1016/j.peptides.2005.07.022

M3 - Article

C2 - 16325965

AN - SCOPUS:33644666434

SN - 0196-9781

VL - 27

SP - 521

EP - 526

JO - Peptides

JF - Peptides

IS - 3

ER -

Identification of PVK/CAP2b neuropeptides from single neurohemal organs of the stable fly and horn fly via MALDI-TOF/TOF tandem mass spectrometry

Abstract

Keywords

ASJC Scopus subject areas

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