Identification of receptors for insulin-like growth factor II in two insulin-like growth factor II producing cell lines

Phillip Lee, D. Hodges, R. L. Hintz, J. H. Wyche, R. G. Rosenfeld

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Specific high affinity membrane receptor(s) for insulin-like growth factor II have been characterized in two cell lines which produce this hormone and have the ability to proliferate in serum-free media. These receptor(s) have no affinity for either insulin or biosynthetic insulin-like growth factor I. Affinity cross-linking and sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed an apparent Mr of 250K which does not change with disulfide bond reduction. Our findings are consistent with an autocrine function for insulin-like growth factor II and indicate that these continuous cell lines may provide unique systems for furthur investigations of this hormone and its receptor.

Original languageEnglish (US)
Pages (from-to)595-600
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume134
Issue number2
DOIs
StatePublished - Jan 29 1986
Externally publishedYes

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IGF Type 2 Receptor
Insulin-Like Growth Factor II
Cells
Hormones
Cell Line
Serum-Free Culture Media
Electrophoresis
Insulin-Like Growth Factor I
Sodium Dodecyl Sulfate
Disulfides
Polyacrylamide Gel Electrophoresis
Insulin
Membranes
polyacrylamide gels

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Identification of receptors for insulin-like growth factor II in two insulin-like growth factor II producing cell lines. / Lee, Phillip; Hodges, D.; Hintz, R. L.; Wyche, J. H.; Rosenfeld, R. G.

In: Biochemical and Biophysical Research Communications, Vol. 134, No. 2, 29.01.1986, p. 595-600.

Research output: Contribution to journalArticle

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