TY - JOUR
T1 - Identification of relevant regions on structural and nonstructural proteins of Zika virus for vaccine and diagnostic test development
T2 - an in silico approach
AU - Salvador, E. A.
AU - Pires de Souza, G. A.
AU - Cotta Malaquias, L. C.
AU - Wang, T.
AU - Leomil Coelho, L. F.
N1 - Publisher Copyright:
© 2019
PY - 2019/5
Y1 - 2019/5
N2 - Zika virus (ZIKV) is an arbovirus belonging to the Flaviviridae family and the genus Flavivirus. Infection with ZIKV causes a mild, self-limiting febrile illness called Zika fever. However, ZIKV infection has been recently associated with microcephaly and Guillain-Barré syndrome. Vaccines for the disease are a high priority of World Health Organization. Several studies are currently being conducted to develop a vaccine against ZIKV, but until now there is no licensed ZIKV vaccine. This study used a novel immunoinformatics approach to identify potential T-cell immunogenic epitopes present in the structural and nonstructural proteins of ZIKV. Fourteen T-cell candidate epitopes were identified on ZIKV structural and nonstructural proteins: pr 36−50 ; C 61−75 ; C 103−117 ; E 374−382 ; E 477−491 ; NS2a 90−104 ; NS2a 174−188 ; NS2a 179−193 ; NS2a 190−204 ; NS2a 195−209 ; NS2a 200−214 ; NS3 175−189 ; and NS4a 82−96 ; NS4a 99−113 . Among these epitopes, only E 374−382 is a human leukocyte antigen (HLA) type I restricted epitope. All identified epitopes showed a low similarity with other important flaviviruses but had a high conservation rate among the ZIKV strains and a high population coverage rate. Therefore, these predicted T-cell epitopes are potential candidates targets for development of vaccines to prevent ZIKV infection.
AB - Zika virus (ZIKV) is an arbovirus belonging to the Flaviviridae family and the genus Flavivirus. Infection with ZIKV causes a mild, self-limiting febrile illness called Zika fever. However, ZIKV infection has been recently associated with microcephaly and Guillain-Barré syndrome. Vaccines for the disease are a high priority of World Health Organization. Several studies are currently being conducted to develop a vaccine against ZIKV, but until now there is no licensed ZIKV vaccine. This study used a novel immunoinformatics approach to identify potential T-cell immunogenic epitopes present in the structural and nonstructural proteins of ZIKV. Fourteen T-cell candidate epitopes were identified on ZIKV structural and nonstructural proteins: pr 36−50 ; C 61−75 ; C 103−117 ; E 374−382 ; E 477−491 ; NS2a 90−104 ; NS2a 174−188 ; NS2a 179−193 ; NS2a 190−204 ; NS2a 195−209 ; NS2a 200−214 ; NS3 175−189 ; and NS4a 82−96 ; NS4a 99−113 . Among these epitopes, only E 374−382 is a human leukocyte antigen (HLA) type I restricted epitope. All identified epitopes showed a low similarity with other important flaviviruses but had a high conservation rate among the ZIKV strains and a high population coverage rate. Therefore, these predicted T-cell epitopes are potential candidates targets for development of vaccines to prevent ZIKV infection.
KW - Diagnostic test
KW - Immunoinformatic
KW - T-cell epitope
KW - Zika virus
KW - vaccine
UR - http://www.scopus.com/inward/record.url?scp=85062015013&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85062015013&partnerID=8YFLogxK
U2 - 10.1016/j.nmni.2019.01.002
DO - 10.1016/j.nmni.2019.01.002
M3 - Article
C2 - 30858979
AN - SCOPUS:85062015013
SN - 2052-2975
VL - 29
JO - New Microbes and New Infections
JF - New Microbes and New Infections
M1 - 100506
ER -