Identification of relevant regions on structural and nonstructural proteins of Zika virus for vaccine and diagnostic test development

an in silico approach

E. A. Salvador, G. A. Pires de Souza, L. C. Cotta Malaquias, Tian Wang, L. F. Leomil Coelho

Research output: Contribution to journalArticle

Abstract

Zika virus (ZIKV) is an arbovirus belonging to the Flaviviridae family and the genus Flavivirus. Infection with ZIKV causes a mild, self-limiting febrile illness called Zika fever. However, ZIKV infection has been recently associated with microcephaly and Guillain-Barré syndrome. Vaccines for the disease are a high priority of World Health Organization. Several studies are currently being conducted to develop a vaccine against ZIKV, but until now there is no licensed ZIKV vaccine. This study used a novel immunoinformatics approach to identify potential T-cell immunogenic epitopes present in the structural and nonstructural proteins of ZIKV. Fourteen T-cell candidate epitopes were identified on ZIKV structural and nonstructural proteins: pr 36−50 ; C 61−75 ; C 103−117 ; E 374−382 ; E 477−491 ; NS2a 90−104 ; NS2a 174−188 ; NS2a 179−193 ; NS2a 190−204 ; NS2a 195−209 ; NS2a 200−214 ; NS3 175−189 ; and NS4a 82−96 ; NS4a 99−113 . Among these epitopes, only E 374−382 is a human leukocyte antigen (HLA) type I restricted epitope. All identified epitopes showed a low similarity with other important flaviviruses but had a high conservation rate among the ZIKV strains and a high population coverage rate. Therefore, these predicted T-cell epitopes are potential candidates targets for development of vaccines to prevent ZIKV infection.

Original languageEnglish (US)
Article number100506
JournalNew Microbes and New Infections
Volume29
DOIs
StatePublished - May 1 2019

Fingerprint

Routine Diagnostic Tests
Computer Simulation
Vaccines
T-Lymphocyte Epitopes
Flavivirus
Epitopes
Proteins
Flaviviridae
Viral Structural Proteins
Arboviruses
Microcephaly
HLA Antigens
Zika Virus
Fever
Zika Virus Infection
Population

Keywords

  • Diagnostic test
  • Immunoinformatic
  • T-cell epitope
  • vaccine
  • Zika virus

ASJC Scopus subject areas

  • Microbiology
  • Infectious Diseases

Cite this

Identification of relevant regions on structural and nonstructural proteins of Zika virus for vaccine and diagnostic test development : an in silico approach. / Salvador, E. A.; Pires de Souza, G. A.; Cotta Malaquias, L. C.; Wang, Tian; Leomil Coelho, L. F.

In: New Microbes and New Infections, Vol. 29, 100506, 01.05.2019.

Research output: Contribution to journalArticle

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