Identification of Rickettsia felis in the salivary glands of cat fleas

Kevin R. Macaluso, Walairat Pornwiroon, Vsevolod Popov, Lane D. Foil

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Rickettsia felis, a flea-associated rickettsial pathogen, has been identified in many tissues, including the digestive and reproductive tissues, within the cat flea, Ctenocephalides felis. We utilized transmission electron microscopy and polymerase chain reaction to identify R. felis in the salivary glands of fed fleas and further define the distribution of R. felis within the arthropod host. We identified Rickettsia-like organisms in salivary glands using electron microscopy. Sequence analysis of portions of the Rickettsia genus-specific 17-kDa antigen gene and R. felis plasmid confirmed the morphological identification of R. felis in cat flea salivary glands. This is the first report of R. felis in tissues critical for horizontal transmission of rickettsiae.

Original languageEnglish (US)
Pages (from-to)391-396
Number of pages6
JournalVector-Borne and Zoonotic Diseases
Volume8
Issue number3
DOIs
StatePublished - Jun 1 2008

Fingerprint

Rickettsia felis
Ctenocephalides
Salivary Glands
Rickettsia
Siphonaptera
Arthropods
R Factors
Transmission Electron Microscopy
Sequence Analysis
Electron Microscopy
Antigens
Polymerase Chain Reaction
Genes

Keywords

  • Cat flea
  • Ctenocephalides felis
  • Rickettsia felis

ASJC Scopus subject areas

  • Infectious Diseases
  • Parasitology

Cite this

Identification of Rickettsia felis in the salivary glands of cat fleas. / Macaluso, Kevin R.; Pornwiroon, Walairat; Popov, Vsevolod; Foil, Lane D.

In: Vector-Borne and Zoonotic Diseases, Vol. 8, No. 3, 01.06.2008, p. 391-396.

Research output: Contribution to journalArticle

Macaluso, Kevin R. ; Pornwiroon, Walairat ; Popov, Vsevolod ; Foil, Lane D. / Identification of Rickettsia felis in the salivary glands of cat fleas. In: Vector-Borne and Zoonotic Diseases. 2008 ; Vol. 8, No. 3. pp. 391-396.
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