Identification of Rickettsia prowazekii using the polymerase chain reaction

L. P. Aniskovich, V. L. Motin, L. J. Lichoded, N. M. Balayeva, G. B. Smirnov

Research output: Contribution to journalArticle

Abstract

Polymerase chain reaction (PCR) was used to identify Rickettsia prowazekii, the etiologic agent of epidemic typhus. For the PCR, Thermus thermophilus thermostable DNA polymerase was applied with buffer containing a relatively low Mg2+ concentration (1.5-2 mM with dNTP's at 250 μM each). A primer pair used to amplify a 448-base-pair (bp) fragment of R. prowazekii genome was Anethesized on the basis of the DNA sequence of gene rpa14/16, coding for a precursor of the mature polypeptides of molecular weight (Mr) 14,000 and/or 16,000 (16kD) from R. prowazekii strain E. For determining the specificity of the primer pair, purified genomic DNAs of 16 rickettsial and 10 other bacterial strains were used.

Original languageEnglish (US)
Pages (from-to)645-649
Number of pages5
JournalEuropean Journal of Epidemiology
Volume9
Issue number6
DOIs
StatePublished - Nov 1 1993

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Keywords

  • Identification
  • PCR
  • R. prowazekii

ASJC Scopus subject areas

  • Epidemiology

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