Identification of Rickettsia prowazekii using the polymerase chain reaction

L. P. Aniskovich, Vladimir Motin, L. J. Lichoded, N. M. Balayeva, G. B. Smirnov

Research output: Contribution to journalArticle

Abstract

Polymerase chain reaction (PCR) was used to identify Rickettsia prowazekii, the etiologic agent of epidemic typhus. For the PCR, Thermus thermophilus thermostable DNA polymerase was applied with buffer containing a relatively low Mg2+ concentration (1.5-2 mM with dNTP's at 250 μM each). A primer pair used to amplify a 448-base-pair (bp) fragment of R. prowazekii genome was Anethesized on the basis of the DNA sequence of gene rpa14/16, coding for a precursor of the mature polypeptides of molecular weight (Mr) 14,000 and/or 16,000 (16kD) from R. prowazekii strain E. For determining the specificity of the primer pair, purified genomic DNAs of 16 rickettsial and 10 other bacterial strains were used.

Original languageEnglish (US)
Pages (from-to)645-649
Number of pages5
JournalEuropean Journal of Epidemiology
Volume9
Issue number6
DOIs
StatePublished - Nov 1993
Externally publishedYes

Fingerprint

Rickettsia prowazekii
polymerase chain reaction
Polymerase Chain Reaction
typhus
Thermus thermophilus
Epidemic Louse-Borne Typhus
DNA-directed DNA polymerase
Base Pairing
polypeptides
Buffers
buffers
Molecular Weight
Genome
molecular weight
genomics
nucleotide sequences
Peptides
genome
DNA
Genes

Keywords

  • Identification
  • PCR
  • R. prowazekii

ASJC Scopus subject areas

  • Epidemiology
  • Agricultural and Biological Sciences(all)

Cite this

Identification of Rickettsia prowazekii using the polymerase chain reaction. / Aniskovich, L. P.; Motin, Vladimir; Lichoded, L. J.; Balayeva, N. M.; Smirnov, G. B.

In: European Journal of Epidemiology, Vol. 9, No. 6, 11.1993, p. 645-649.

Research output: Contribution to journalArticle

Aniskovich, L. P. ; Motin, Vladimir ; Lichoded, L. J. ; Balayeva, N. M. ; Smirnov, G. B. / Identification of Rickettsia prowazekii using the polymerase chain reaction. In: European Journal of Epidemiology. 1993 ; Vol. 9, No. 6. pp. 645-649.
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