Identification of rickettsiae in cutaneous biopsy specimens from dogs with experimental Rocky Mountain spotted fever.

M. G. Davidson, E. B. Breitschwerdt, D. H. Walker, M. P. Nasisse, W. E. Sussman

Research output: Contribution to journalArticle

Abstract

Direct immunofluorescence reaction for Rickettsia rickettsii was performed on formalin-fixed, paraffin-embedded cutaneous biopsy specimens collected from dogs with experimental Rocky Mountain spotted fever (RMSF). A technique of trypsin digestion of deparaffinized, rehydrated sections was successful in demonstrating discrete, immunofluorescent organisms in endothelia and adjacent vessel walls in the dermis. R rickettsii was identified only in grossly evident dermal lesions (macular rash or oral vesicles) and was not apparent in randomly collected biopsy specimens from clinically normal inguinal skin. These results suggest that clinical application of this technique for diagnosis of RMSF may be limited in dogs without cutaneous lesions.

Original languageEnglish (US)
Pages (from-to)8-11
Number of pages4
JournalJournal of veterinary internal medicine / American College of Veterinary Internal Medicine
Volume3
Issue number1
StatePublished - Jan 1989
Externally publishedYes

Fingerprint

Rocky Mountain spotted fever
Rocky Mountain Spotted Fever
Rickettsia
biopsy
Rickettsia rickettsii
Dogs
Biopsy
Skin
dogs
dermis
skin lesions
endothelium
formalin
skin (animal)
lesions (animal)
alkanes
trypsin
fluorescent antibody technique
mouth
digestion

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Identification of rickettsiae in cutaneous biopsy specimens from dogs with experimental Rocky Mountain spotted fever. / Davidson, M. G.; Breitschwerdt, E. B.; Walker, D. H.; Nasisse, M. P.; Sussman, W. E.

In: Journal of veterinary internal medicine / American College of Veterinary Internal Medicine, Vol. 3, No. 1, 01.1989, p. 8-11.

Research output: Contribution to journalArticle

@article{8a1ed9a902984a9eae75e0dc33fcb984,
title = "Identification of rickettsiae in cutaneous biopsy specimens from dogs with experimental Rocky Mountain spotted fever.",
abstract = "Direct immunofluorescence reaction for Rickettsia rickettsii was performed on formalin-fixed, paraffin-embedded cutaneous biopsy specimens collected from dogs with experimental Rocky Mountain spotted fever (RMSF). A technique of trypsin digestion of deparaffinized, rehydrated sections was successful in demonstrating discrete, immunofluorescent organisms in endothelia and adjacent vessel walls in the dermis. R rickettsii was identified only in grossly evident dermal lesions (macular rash or oral vesicles) and was not apparent in randomly collected biopsy specimens from clinically normal inguinal skin. These results suggest that clinical application of this technique for diagnosis of RMSF may be limited in dogs without cutaneous lesions.",
author = "Davidson, {M. G.} and Breitschwerdt, {E. B.} and Walker, {D. H.} and Nasisse, {M. P.} and Sussman, {W. E.}",
year = "1989",
month = "1",
language = "English (US)",
volume = "3",
pages = "8--11",
journal = "Journal of Veterinary Internal Medicine",
issn = "0891-6640",
publisher = "Wiley-Blackwell",
number = "1",

}

TY - JOUR

T1 - Identification of rickettsiae in cutaneous biopsy specimens from dogs with experimental Rocky Mountain spotted fever.

AU - Davidson, M. G.

AU - Breitschwerdt, E. B.

AU - Walker, D. H.

AU - Nasisse, M. P.

AU - Sussman, W. E.

PY - 1989/1

Y1 - 1989/1

N2 - Direct immunofluorescence reaction for Rickettsia rickettsii was performed on formalin-fixed, paraffin-embedded cutaneous biopsy specimens collected from dogs with experimental Rocky Mountain spotted fever (RMSF). A technique of trypsin digestion of deparaffinized, rehydrated sections was successful in demonstrating discrete, immunofluorescent organisms in endothelia and adjacent vessel walls in the dermis. R rickettsii was identified only in grossly evident dermal lesions (macular rash or oral vesicles) and was not apparent in randomly collected biopsy specimens from clinically normal inguinal skin. These results suggest that clinical application of this technique for diagnosis of RMSF may be limited in dogs without cutaneous lesions.

AB - Direct immunofluorescence reaction for Rickettsia rickettsii was performed on formalin-fixed, paraffin-embedded cutaneous biopsy specimens collected from dogs with experimental Rocky Mountain spotted fever (RMSF). A technique of trypsin digestion of deparaffinized, rehydrated sections was successful in demonstrating discrete, immunofluorescent organisms in endothelia and adjacent vessel walls in the dermis. R rickettsii was identified only in grossly evident dermal lesions (macular rash or oral vesicles) and was not apparent in randomly collected biopsy specimens from clinically normal inguinal skin. These results suggest that clinical application of this technique for diagnosis of RMSF may be limited in dogs without cutaneous lesions.

UR - http://www.scopus.com/inward/record.url?scp=0024486564&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024486564&partnerID=8YFLogxK

M3 - Article

VL - 3

SP - 8

EP - 11

JO - Journal of Veterinary Internal Medicine

JF - Journal of Veterinary Internal Medicine

SN - 0891-6640

IS - 1

ER -