Identification of Rickettsiae in Cutaneous i Biopsy Specimens From Dogs With Experimental Rocky Mountain Spotted Fever

Michael G. Davidson, Edward B. Breitschwerdt, David Walker, Mark P. Nasisse, Wendy E. Sussman

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Direct immunofluorescence reaction for Rickettsia rickettsii was performed on formalin‐fixed, paraffin‐embedded cutaneous biopsy specimens collected from dogs with experimental Rocky Mountain spotted fever (RMSF). A technique of trypsin digestion of deparaffinized, rehydrated sections was successful in demonstrating discrete, immunofluorescent organisms in endothelia and adjacent vessel walls in the dermis. R rickettsii was identified only in grossly evident dermal lesions (macular rash or oral vesicles) and was not apparent in randomly collected biopsy specimens from clinically normal inguinal skin. These results suggest that clinical application of this technique for diagnosis of RMSF may be limited in dogs without cutaneous lesions. (Journal of Veterinary Internal Medicine 1989; 3:8–11)

Original languageEnglish (US)
Pages (from-to)7-11
Number of pages5
JournalJournal of Veterinary Internal Medicine
Volume3
Issue number1
DOIs
StatePublished - 1989
Externally publishedYes

Fingerprint

Rocky Mountain spotted fever
Rocky Mountain Spotted Fever
Rickettsia
biopsy
Rickettsia rickettsii
Dogs
Biopsy
Skin
dogs
dermis
skin lesions
endothelium
skin (animal)
lesions (animal)
trypsin
fluorescent antibody technique
mouth
medicine
digestion
Direct Fluorescent Antibody Technique

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Identification of Rickettsiae in Cutaneous i Biopsy Specimens From Dogs With Experimental Rocky Mountain Spotted Fever. / Davidson, Michael G.; Breitschwerdt, Edward B.; Walker, David; Nasisse, Mark P.; Sussman, Wendy E.

In: Journal of Veterinary Internal Medicine, Vol. 3, No. 1, 1989, p. 7-11.

Research output: Contribution to journalArticle

Davidson, Michael G. ; Breitschwerdt, Edward B. ; Walker, David ; Nasisse, Mark P. ; Sussman, Wendy E. / Identification of Rickettsiae in Cutaneous i Biopsy Specimens From Dogs With Experimental Rocky Mountain Spotted Fever. In: Journal of Veterinary Internal Medicine. 1989 ; Vol. 3, No. 1. pp. 7-11.
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