TY - JOUR
T1 - Identification of serum biomarkers for aging and anabolic response
AU - Banerjee, Camellia
AU - Ulloor, Jagadish
AU - Dillon, Edgar L.
AU - Dahodwala, Qusai
AU - Franklin, Brittani
AU - Storer, Thomas
AU - Sebastiani, Paola
AU - Sheffield-Moore, Melinda
AU - Urban, Randall J.
AU - Bhasin, Shalender
AU - Montano, Monty
N1 - Funding Information:
The authors wish to acknowledge support from the Boston OAIC Pepper Center and NIAMS R01 AR055115 (MM). This work was supported in part by the UTMB Claude D. Pepper Older Americans Independence Center NIH/NIA Grant # P30 AG024832 and also supported in part by the UTMB Institute for Translational Sciences - Clinical Research Center (ITS-CRC) grant 1UL1RR029876-01 from the National Center for Research Resources, National Institutes of Health.
PY - 2011/6/20
Y1 - 2011/6/20
N2 - Objective: With the progressive aging of the human population, there is an inexorable decline in muscle mass, strength and function. Anabolic supplementation with testosterone has been shown to effectively restore muscle mass in both young and elderly men. In this study, we were interested in identifying serum factors that change with age in two distinct age groups of healthy men, and whether these factors were affected by testosterone supplementation.Methods: We measured the protein levels of a number of serum biomarkers using a combination of banked serum samples from older men (60 to 75 years) and younger men (ages 18 to 35), as well as new serum specimens obtained through collaboration. We compared baseline levels of all biomarkers between young and older men. In addition, we evaluated potential changes in these biomarker levels in association with testosterone dose (low dose defined as 125 mg per week or below compared to high dose defined as 300 mg per week or above) in our banked specimens.Results: We identified nine serum biomarkers that differed between the young and older subjects. These age-associated biomarkers included: insulin-like growth factor (IGF1), N-terminal propeptide of type III collagen (PIIINP), monokine induced by gamma interferon (MIG), epithelial-derived neutrophil-activating peptide 78 (ENA78), interleukin 7 (IL-7), p40 subunit of interleukin 12 (IL-12p40), macrophage inflammatory protein 1β (MIP-1β), platelet derived growth factor β (PDGFβ) and interferon-inducible protein 10 (IP-10). We further observed testosterone dose-associated changes in some but not all age related markers: IGF1, PIIINP, leptin, MIG and ENA78. Gains in lean mass were confirmed by dual energy X-ray absorptiometry (DEXA).Conclusions: Results from this study suggest that there are potential phenotypic biomarkers in serum that can be associated with healthy aging and that some but not all of these biomarkers reflect gains in muscle mass upon testosterone administration.
AB - Objective: With the progressive aging of the human population, there is an inexorable decline in muscle mass, strength and function. Anabolic supplementation with testosterone has been shown to effectively restore muscle mass in both young and elderly men. In this study, we were interested in identifying serum factors that change with age in two distinct age groups of healthy men, and whether these factors were affected by testosterone supplementation.Methods: We measured the protein levels of a number of serum biomarkers using a combination of banked serum samples from older men (60 to 75 years) and younger men (ages 18 to 35), as well as new serum specimens obtained through collaboration. We compared baseline levels of all biomarkers between young and older men. In addition, we evaluated potential changes in these biomarker levels in association with testosterone dose (low dose defined as 125 mg per week or below compared to high dose defined as 300 mg per week or above) in our banked specimens.Results: We identified nine serum biomarkers that differed between the young and older subjects. These age-associated biomarkers included: insulin-like growth factor (IGF1), N-terminal propeptide of type III collagen (PIIINP), monokine induced by gamma interferon (MIG), epithelial-derived neutrophil-activating peptide 78 (ENA78), interleukin 7 (IL-7), p40 subunit of interleukin 12 (IL-12p40), macrophage inflammatory protein 1β (MIP-1β), platelet derived growth factor β (PDGFβ) and interferon-inducible protein 10 (IP-10). We further observed testosterone dose-associated changes in some but not all age related markers: IGF1, PIIINP, leptin, MIG and ENA78. Gains in lean mass were confirmed by dual energy X-ray absorptiometry (DEXA).Conclusions: Results from this study suggest that there are potential phenotypic biomarkers in serum that can be associated with healthy aging and that some but not all of these biomarkers reflect gains in muscle mass upon testosterone administration.
KW - Age
KW - Biomarker
KW - Testosterone
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U2 - 10.1186/1742-4933-8-5
DO - 10.1186/1742-4933-8-5
M3 - Article
C2 - 21689392
AN - SCOPUS:79959214454
SN - 1742-4933
VL - 8
JO - Immunity and Ageing
JF - Immunity and Ageing
M1 - 5
ER -