Identification of spinothalamic tract cells in fresh, unfixed rat spinal cord

Li Yen Mae Huang, Susan M. Carlton, William D. Willis

Research output: Contribution to journalArticlepeer-review

4 Scopus citations


Diamidino yellow dihydrochloride (DY·2HCl) can reliably label spinothalamic tract cells both in the perfused, fixed and in the fresh, unfixed rat spinal cord. The major advantages of DY·2HCl as a retrograde marker are that it leaks from the cells very slowly and can be conveniently identified in unfixed neurons by fluorescence microscopy. DY·2HCl will be a useful marker for identification of spinothalamic tract cells that are to be studied in vitro.

Original languageEnglish (US)
Pages (from-to)91-96
Number of pages6
JournalJournal of Neuroscience Methods
Issue number2
StatePublished - Jul 1985


  • cell identification
  • diamidino yellow dihydrochloride
  • fluorescent label
  • fresh
  • rat spinal cord
  • retrograde marker
  • spinothalamic tract cell
  • unfixed spinal cord

ASJC Scopus subject areas

  • General Neuroscience


Dive into the research topics of 'Identification of spinothalamic tract cells in fresh, unfixed rat spinal cord'. Together they form a unique fingerprint.

Cite this