Abstract
Diamidino yellow dihydrochloride (DY·2HCl) can reliably label spinothalamic tract cells both in the perfused, fixed and in the fresh, unfixed rat spinal cord. The major advantages of DY·2HCl as a retrograde marker are that it leaks from the cells very slowly and can be conveniently identified in unfixed neurons by fluorescence microscopy. DY·2HCl will be a useful marker for identification of spinothalamic tract cells that are to be studied in vitro.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 91-96 |
| Number of pages | 6 |
| Journal | Journal of Neuroscience Methods |
| Volume | 14 |
| Issue number | 2 |
| DOIs | |
| State | Published - Jul 1985 |
Keywords
- cell identification
- diamidino yellow dihydrochloride
- fluorescent label
- fresh
- rat spinal cord
- retrograde marker
- spinothalamic tract cell
- unfixed spinal cord
ASJC Scopus subject areas
- General Neuroscience
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