Identification of the first neuropeptides from the CNS of Hemiptera: CAPA peptides of the southern green stinkbug Nezara viridula (L.)

Reinhard Predel, William Russell, Susanne Neupert, David H. Russell, Jesus F. Esquivel, Ronald J. Nachman

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

A direct mass spectrometric investigation of nerve homologs of the abdominal perisympathetic organs was employed to reveal the first and complete sequences of CAPA peptides from a hemipteran species, the southern green stinkbug Nezara viridula. Side-chain fragmentations allowed the assignment of internal Leu/Ile; on-plate acetylation was used to distinguish between the mass-related Lys and Gln. The following sequences were obtained: DQLFPFPRV-NH2 (CAPA-PVK-1), EQLIPFPRV-NH2 (CAPA-PVK-2), and NGSAGNGGLWFGPRL/I-NH2 (CAPA-PK). CAPA-PVKs are associated with the regulation of diuresis in insects, and identification of those native to a hemipteran will provide the experimental basis to better understand regulation of water balance in this family of insects.

Original languageEnglish (US)
Pages (from-to)2670-2677
Number of pages8
JournalPeptides
Volume27
Issue number11
DOIs
StatePublished - Nov 2006
Externally publishedYes

Fingerprint

Acetylation
Hemiptera
Neuropeptides
Insects
Peptides
Water
Diuresis

Keywords

  • CAP2b
  • CAPA gene
  • FXPRLamide
  • Insect neuropeptide
  • Mass spectrometry
  • Perisympathetic organs
  • Periviscerokinin
  • Pyrokinin

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Physiology
  • Cellular and Molecular Neuroscience

Cite this

Identification of the first neuropeptides from the CNS of Hemiptera : CAPA peptides of the southern green stinkbug Nezara viridula (L.). / Predel, Reinhard; Russell, William; Neupert, Susanne; Russell, David H.; Esquivel, Jesus F.; Nachman, Ronald J.

In: Peptides, Vol. 27, No. 11, 11.2006, p. 2670-2677.

Research output: Contribution to journalArticle

Predel, Reinhard ; Russell, William ; Neupert, Susanne ; Russell, David H. ; Esquivel, Jesus F. ; Nachman, Ronald J. / Identification of the first neuropeptides from the CNS of Hemiptera : CAPA peptides of the southern green stinkbug Nezara viridula (L.). In: Peptides. 2006 ; Vol. 27, No. 11. pp. 2670-2677.
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