Identification of the first neuropeptides from the CNS of Hemiptera: CAPA peptides of the southern green stinkbug Nezara viridula (L.)

Reinhard Predel, William K. Russell, Susanne Neupert, David H. Russell, Jesus F. Esquivel, Ronald J. Nachman

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

A direct mass spectrometric investigation of nerve homologs of the abdominal perisympathetic organs was employed to reveal the first and complete sequences of CAPA peptides from a hemipteran species, the southern green stinkbug Nezara viridula. Side-chain fragmentations allowed the assignment of internal Leu/Ile; on-plate acetylation was used to distinguish between the mass-related Lys and Gln. The following sequences were obtained: DQLFPFPRV-NH2 (CAPA-PVK-1), EQLIPFPRV-NH2 (CAPA-PVK-2), and NGSAGNGGLWFGPRL/I-NH2 (CAPA-PK). CAPA-PVKs are associated with the regulation of diuresis in insects, and identification of those native to a hemipteran will provide the experimental basis to better understand regulation of water balance in this family of insects.

Original languageEnglish (US)
Pages (from-to)2670-2677
Number of pages8
JournalPeptides
Volume27
Issue number11
DOIs
StatePublished - Nov 1 2006
Externally publishedYes

Keywords

  • CAP2b
  • CAPA gene
  • FXPRLamide
  • Insect neuropeptide
  • Mass spectrometry
  • Perisympathetic organs
  • Periviscerokinin
  • Pyrokinin

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Endocrinology
  • Cellular and Molecular Neuroscience

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