Identification of the first neuropeptides from the CNS of Hemiptera

CAPA peptides of the southern green stinkbug Nezara viridula (L.)

Reinhard Predel, William Russell, Susanne Neupert, David H. Russell, Jesus F. Esquivel, Ronald J. Nachman

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

A direct mass spectrometric investigation of nerve homologs of the abdominal perisympathetic organs was employed to reveal the first and complete sequences of CAPA peptides from a hemipteran species, the southern green stinkbug Nezara viridula. Side-chain fragmentations allowed the assignment of internal Leu/Ile; on-plate acetylation was used to distinguish between the mass-related Lys and Gln. The following sequences were obtained: DQLFPFPRV-NH2 (CAPA-PVK-1), EQLIPFPRV-NH2 (CAPA-PVK-2), and NGSAGNGGLWFGPRL/I-NH2 (CAPA-PK). CAPA-PVKs are associated with the regulation of diuresis in insects, and identification of those native to a hemipteran will provide the experimental basis to better understand regulation of water balance in this family of insects.

Original languageEnglish (US)
Pages (from-to)2670-2677
Number of pages8
JournalPeptides
Volume27
Issue number11
DOIs
StatePublished - Nov 2006
Externally publishedYes

Fingerprint

Acetylation
Hemiptera
Neuropeptides
Insects
Peptides
Water
Diuresis

Keywords

  • CAP2b
  • CAPA gene
  • FXPRLamide
  • Insect neuropeptide
  • Mass spectrometry
  • Perisympathetic organs
  • Periviscerokinin
  • Pyrokinin

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Physiology
  • Cellular and Molecular Neuroscience

Cite this

Identification of the first neuropeptides from the CNS of Hemiptera : CAPA peptides of the southern green stinkbug Nezara viridula (L.). / Predel, Reinhard; Russell, William; Neupert, Susanne; Russell, David H.; Esquivel, Jesus F.; Nachman, Ronald J.

In: Peptides, Vol. 27, No. 11, 11.2006, p. 2670-2677.

Research output: Contribution to journalArticle

Predel, Reinhard ; Russell, William ; Neupert, Susanne ; Russell, David H. ; Esquivel, Jesus F. ; Nachman, Ronald J. / Identification of the first neuropeptides from the CNS of Hemiptera : CAPA peptides of the southern green stinkbug Nezara viridula (L.). In: Peptides. 2006 ; Vol. 27, No. 11. pp. 2670-2677.
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