Identification of the first neuropeptides from the CNS of Hemiptera: CAPA peptides of the southern green stinkbug Nezara viridula (L.)

Reinhard Predel; William K. Russell; Susanne Neupert; David H. Russell; Jesus F. Esquivel; Ronald J. Nachman

doi:10.1016/j.peptides.2006.05.013

Identification of the first neuropeptides from the CNS of Hemiptera: CAPA peptides of the southern green stinkbug Nezara viridula (L.)

Reinhard Predel, William K. Russell, Susanne Neupert, David H. Russell, Jesus F. Esquivel, Ronald J. Nachman

Research output: Contribution to journal › Article › peer-review

19 Scopus citations

Abstract

A direct mass spectrometric investigation of nerve homologs of the abdominal perisympathetic organs was employed to reveal the first and complete sequences of CAPA peptides from a hemipteran species, the southern green stinkbug Nezara viridula. Side-chain fragmentations allowed the assignment of internal Leu/Ile; on-plate acetylation was used to distinguish between the mass-related Lys and Gln. The following sequences were obtained: DQLFPFPRV-NH₂ (CAPA-PVK-1), EQLIPFPRV-NH₂ (CAPA-PVK-2), and NGSAGNGGLWFGPRL/I-NH₂ (CAPA-PK). CAPA-PVKs are associated with the regulation of diuresis in insects, and identification of those native to a hemipteran will provide the experimental basis to better understand regulation of water balance in this family of insects.

Original language	English (US)
Pages (from-to)	2670-2677
Number of pages	8
Journal	Peptides
Volume	27
Issue number	11
DOIs	https://doi.org/10.1016/j.peptides.2006.05.013
State	Published - Nov 2006
Externally published	Yes

Keywords

CAP2b
CAPA gene
FXPRLamide
Insect neuropeptide
Mass spectrometry
Perisympathetic organs
Periviscerokinin
Pyrokinin

ASJC Scopus subject areas

Biochemistry
Physiology
Endocrinology
Cellular and Molecular Neuroscience

Access to Document

10.1016/j.peptides.2006.05.013

Cite this

@article{6bf79ffea72a4c16b83cecb67528406b,

title = "Identification of the first neuropeptides from the CNS of Hemiptera: CAPA peptides of the southern green stinkbug Nezara viridula (L.)",

abstract = "A direct mass spectrometric investigation of nerve homologs of the abdominal perisympathetic organs was employed to reveal the first and complete sequences of CAPA peptides from a hemipteran species, the southern green stinkbug Nezara viridula. Side-chain fragmentations allowed the assignment of internal Leu/Ile; on-plate acetylation was used to distinguish between the mass-related Lys and Gln. The following sequences were obtained: DQLFPFPRV-NH2 (CAPA-PVK-1), EQLIPFPRV-NH2 (CAPA-PVK-2), and NGSAGNGGLWFGPRL/I-NH2 (CAPA-PK). CAPA-PVKs are associated with the regulation of diuresis in insects, and identification of those native to a hemipteran will provide the experimental basis to better understand regulation of water balance in this family of insects.",

keywords = "CAP2b, CAPA gene, FXPRLamide, Insect neuropeptide, Mass spectrometry, Perisympathetic organs, Periviscerokinin, Pyrokinin",

author = "Reinhard Predel and Russell, {William K.} and Susanne Neupert and Russell, {David H.} and Esquivel, {Jesus F.} and Nachman, {Ronald J.}",

note = "Funding Information: This study was supported with a grant from Deutsche Forschungsgemeinschaft (Predel 595/6-1,2), a Binational Agricultural Research and Development Grant (BARD #3356-02)(RJN) and a Collaborative Research Grant (#LST.CLG.979226) from the North Atlantic Treaty Organization (NATO) (RJN), and grants provided to the Laboratory for Biological Mass Spectrometry at Texas A&M University, College Station: R01 RR019587 NIH and DE-FG02-04ER15520 DOE. In addition, we acknowledge the capable technical assistance of Allison Strey, Pawel Zubrzak and Nan Pryor of the USDA Areawide Pest Management Research Unit, Southern Plains Agricultural Research Center.",

year = "2006",

month = nov,

doi = "10.1016/j.peptides.2006.05.013",

language = "English (US)",

volume = "27",

pages = "2670--2677",

journal = "Peptides",

issn = "0196-9781",

publisher = "Elsevier Inc.",

number = "11",

}

TY - JOUR

T1 - Identification of the first neuropeptides from the CNS of Hemiptera

T2 - CAPA peptides of the southern green stinkbug Nezara viridula (L.)

AU - Predel, Reinhard

AU - Russell, William K.

AU - Neupert, Susanne

AU - Russell, David H.

AU - Esquivel, Jesus F.

AU - Nachman, Ronald J.

N1 - Funding Information: This study was supported with a grant from Deutsche Forschungsgemeinschaft (Predel 595/6-1,2), a Binational Agricultural Research and Development Grant (BARD #3356-02)(RJN) and a Collaborative Research Grant (#LST.CLG.979226) from the North Atlantic Treaty Organization (NATO) (RJN), and grants provided to the Laboratory for Biological Mass Spectrometry at Texas A&M University, College Station: R01 RR019587 NIH and DE-FG02-04ER15520 DOE. In addition, we acknowledge the capable technical assistance of Allison Strey, Pawel Zubrzak and Nan Pryor of the USDA Areawide Pest Management Research Unit, Southern Plains Agricultural Research Center.

PY - 2006/11

Y1 - 2006/11

N2 - A direct mass spectrometric investigation of nerve homologs of the abdominal perisympathetic organs was employed to reveal the first and complete sequences of CAPA peptides from a hemipteran species, the southern green stinkbug Nezara viridula. Side-chain fragmentations allowed the assignment of internal Leu/Ile; on-plate acetylation was used to distinguish between the mass-related Lys and Gln. The following sequences were obtained: DQLFPFPRV-NH2 (CAPA-PVK-1), EQLIPFPRV-NH2 (CAPA-PVK-2), and NGSAGNGGLWFGPRL/I-NH2 (CAPA-PK). CAPA-PVKs are associated with the regulation of diuresis in insects, and identification of those native to a hemipteran will provide the experimental basis to better understand regulation of water balance in this family of insects.

AB - A direct mass spectrometric investigation of nerve homologs of the abdominal perisympathetic organs was employed to reveal the first and complete sequences of CAPA peptides from a hemipteran species, the southern green stinkbug Nezara viridula. Side-chain fragmentations allowed the assignment of internal Leu/Ile; on-plate acetylation was used to distinguish between the mass-related Lys and Gln. The following sequences were obtained: DQLFPFPRV-NH2 (CAPA-PVK-1), EQLIPFPRV-NH2 (CAPA-PVK-2), and NGSAGNGGLWFGPRL/I-NH2 (CAPA-PK). CAPA-PVKs are associated with the regulation of diuresis in insects, and identification of those native to a hemipteran will provide the experimental basis to better understand regulation of water balance in this family of insects.

KW - CAP2b

KW - CAPA gene

KW - FXPRLamide

KW - Insect neuropeptide

KW - Mass spectrometry

KW - Perisympathetic organs

KW - Periviscerokinin

KW - Pyrokinin

UR - http://www.scopus.com/inward/record.url?scp=33750053907&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33750053907&partnerID=8YFLogxK

U2 - 10.1016/j.peptides.2006.05.013

DO - 10.1016/j.peptides.2006.05.013

M3 - Article

C2 - 16824649

AN - SCOPUS:33750053907

SN - 0196-9781

VL - 27

SP - 2670

EP - 2677

JO - Peptides

JF - Peptides

IS - 11

ER -

Identification of the first neuropeptides from the CNS of Hemiptera: CAPA peptides of the southern green stinkbug Nezara viridula (L.)

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this