TY - JOUR
T1 - Identification of the target cells of Orientia tsutsugamushi in human cases of scrub typhus
AU - Moron, Cecilia G.
AU - Popov, Vsevolod L.
AU - Feng, Hui Min
AU - Wear, Douglas
AU - Walker, David H.
N1 - Funding Information:
Copyright © 2001 by The United States and Canadian Academy of Pathology, Inc. VOL. 14, NO. 8, P. 752, 2001 Printed in the U.S.A. Date of acceptance: February 27, 2001. This work was supported by a Fogarty Grant for International Training and Research in Emerging Infectious Diseases (ITREID). Address reprint requests to: David H. Walker, M.D., Department of Pathology, University of Texas Medical Branch, 301 University Blvd., Galveston, TX 77555-0609; e-mail: [email protected]; fax: 409-772-2500.
PY - 2001
Y1 - 2001
N2 - Orientia tsutsugamushi is the etiologic agent of scrub typhus, a chigger-borne zoonosis that is a highly prevalent, life-threatening illness of greatest public health importance in tropical Asia and the islands of the western Pacific Ocean. The target cell of this bacterium is poorly defined in humans. In this study, O. tsutsugamushi were identified by immunohistochemistry using a rabbit polyclonal antibody raised against O. tsutsugamushi Karp strain in paraffin-embedded archived autopsy tissues of three patients with clinical suspicion of scrub typhus who died during World War II and the Vietnam War. Rickettsiae were located in endothelial cells in all of the organs evaluated, namely heart, lung, brain, kidney, pancreas, and skin, and within cardiac muscle cells and in macrophages located in liver and spleen. Electron microscopy confirmed the location of rickettsiae in endothelium and cardiac myocytes.
AB - Orientia tsutsugamushi is the etiologic agent of scrub typhus, a chigger-borne zoonosis that is a highly prevalent, life-threatening illness of greatest public health importance in tropical Asia and the islands of the western Pacific Ocean. The target cell of this bacterium is poorly defined in humans. In this study, O. tsutsugamushi were identified by immunohistochemistry using a rabbit polyclonal antibody raised against O. tsutsugamushi Karp strain in paraffin-embedded archived autopsy tissues of three patients with clinical suspicion of scrub typhus who died during World War II and the Vietnam War. Rickettsiae were located in endothelial cells in all of the organs evaluated, namely heart, lung, brain, kidney, pancreas, and skin, and within cardiac muscle cells and in macrophages located in liver and spleen. Electron microscopy confirmed the location of rickettsiae in endothelium and cardiac myocytes.
KW - Immunohistochemistry
KW - Orientia tsutsugamushi
KW - Rickettsia, Scrub typhus
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U2 - 10.1038/modpathol.3880385
DO - 10.1038/modpathol.3880385
M3 - Article
C2 - 11504834
AN - SCOPUS:0035162792
SN - 0893-3952
VL - 14
SP - 752
EP - 759
JO - Modern Pathology
JF - Modern Pathology
IS - 8
ER -