Identification of the target cells of Orientia tsutsugamushi in human cases of scrub typhus

Cecilia G. Moron, Vsevolod Popov, Hui Min Feng, Douglas Wear, David Walker

Research output: Contribution to journalArticle

102 Citations (Scopus)

Abstract

Orientia tsutsugamushi is the etiologic agent of scrub typhus, a chigger-borne zoonosis that is a highly prevalent, life-threatening illness of greatest public health importance in tropical Asia and the islands of the western Pacific Ocean. The target cell of this bacterium is poorly defined in humans. In this study, O. tsutsugamushi were identified by immunohistochemistry using a rabbit polyclonal antibody raised against O. tsutsugamushi Karp strain in paraffin-embedded archived autopsy tissues of three patients with clinical suspicion of scrub typhus who died during World War II and the Vietnam War. Rickettsiae were located in endothelial cells in all of the organs evaluated, namely heart, lung, brain, kidney, pancreas, and skin, and within cardiac muscle cells and in macrophages located in liver and spleen. Electron microscopy confirmed the location of rickettsiae in endothelium and cardiac myocytes.

Original languageEnglish (US)
Pages (from-to)752-759
Number of pages8
JournalModern Pathology
Volume14
Issue number8
DOIs
StatePublished - 2001

Fingerprint

Orientia tsutsugamushi
Scrub Typhus
Rickettsia
Cardiac Myocytes
Western Asia
Trombiculidae
Pacific Ocean
World War II
Vietnam
Zoonoses
Islands
Paraffin
Endothelium
Pancreas
Autopsy
Electron Microscopy
Spleen
Endothelial Cells
Public Health
Immunohistochemistry

Keywords

  • Immunohistochemistry
  • Orientia tsutsugamushi
  • Rickettsia, Scrub typhus

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Identification of the target cells of Orientia tsutsugamushi in human cases of scrub typhus. / Moron, Cecilia G.; Popov, Vsevolod; Feng, Hui Min; Wear, Douglas; Walker, David.

In: Modern Pathology, Vol. 14, No. 8, 2001, p. 752-759.

Research output: Contribution to journalArticle

Moron, Cecilia G. ; Popov, Vsevolod ; Feng, Hui Min ; Wear, Douglas ; Walker, David. / Identification of the target cells of Orientia tsutsugamushi in human cases of scrub typhus. In: Modern Pathology. 2001 ; Vol. 14, No. 8. pp. 752-759.
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