Identification of thioredoxin-2 as a regulator of the mitochondrial permeability transition

Min He; Jiyang Cai; Young Mi Go; Jennifer M. Johnson; W. David Martin; Jason M. Hansen; Dean P. Jones

doi:10.1093/toxsci/kfn116

Identification of thioredoxin-2 as a regulator of the mitochondrial permeability transition

Min He, Jiyang Cai, Young Mi Go, Jennifer M. Johnson, W. David Martin, Jason M. Hansen, Dean P. Jones

Research output: Contribution to journal › Article › peer-review

44 Scopus citations

Abstract

Thioredoxin-2 (Trx2) is a multifunctional, mitochondria-specific protein, which inhibits cell death. The mitochondrial permeability transition (MPT) is a distinct mechanism for cell death activated by oxidants and linked to both necrotic and apoptotic morphologies. We studied mitochondria from Trx2 transgenic mice to determine whether Trx2 protects against oxidant-induced MPT. All experiments were performed in isolated mitochondria. Results showed that Trx2 protected against MPT induced by exogenously added peroxide. Unexpectedly, Trx2 also protected against the MPT induced by Ca²⁺ in the absence of added peroxide. The results indicate that in addition to protecting against oxidative stress, Trx2 is an endogenous regulator of the MPT.

Original language	English (US)
Pages (from-to)	44-50
Number of pages	7
Journal	Toxicological Sciences
Volume	105
Issue number	1
DOIs	https://doi.org/10.1093/toxsci/kfn116
State	Published - 2008
Externally published	Yes

Keywords

Apoptosis
Calcium
Cell death mechanisms
Necrosis
Transgenic mice

ASJC Scopus subject areas

Toxicology

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10.1093/toxsci/kfn116

Cite this

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title = "Identification of thioredoxin-2 as a regulator of the mitochondrial permeability transition",

abstract = "Thioredoxin-2 (Trx2) is a multifunctional, mitochondria-specific protein, which inhibits cell death. The mitochondrial permeability transition (MPT) is a distinct mechanism for cell death activated by oxidants and linked to both necrotic and apoptotic morphologies. We studied mitochondria from Trx2 transgenic mice to determine whether Trx2 protects against oxidant-induced MPT. All experiments were performed in isolated mitochondria. Results showed that Trx2 protected against MPT induced by exogenously added peroxide. Unexpectedly, Trx2 also protected against the MPT induced by Ca2+ in the absence of added peroxide. The results indicate that in addition to protecting against oxidative stress, Trx2 is an endogenous regulator of the MPT.",

keywords = "Apoptosis, Calcium, Cell death mechanisms, Necrosis, Transgenic mice",

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doi = "10.1093/toxsci/kfn116",

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T1 - Identification of thioredoxin-2 as a regulator of the mitochondrial permeability transition

AU - He, Min

AU - Cai, Jiyang

AU - Go, Young Mi

AU - Johnson, Jennifer M.

AU - Martin, W. David

AU - Hansen, Jason M.

AU - Jones, Dean P.

PY - 2008

Y1 - 2008

N2 - Thioredoxin-2 (Trx2) is a multifunctional, mitochondria-specific protein, which inhibits cell death. The mitochondrial permeability transition (MPT) is a distinct mechanism for cell death activated by oxidants and linked to both necrotic and apoptotic morphologies. We studied mitochondria from Trx2 transgenic mice to determine whether Trx2 protects against oxidant-induced MPT. All experiments were performed in isolated mitochondria. Results showed that Trx2 protected against MPT induced by exogenously added peroxide. Unexpectedly, Trx2 also protected against the MPT induced by Ca2+ in the absence of added peroxide. The results indicate that in addition to protecting against oxidative stress, Trx2 is an endogenous regulator of the MPT.

AB - Thioredoxin-2 (Trx2) is a multifunctional, mitochondria-specific protein, which inhibits cell death. The mitochondrial permeability transition (MPT) is a distinct mechanism for cell death activated by oxidants and linked to both necrotic and apoptotic morphologies. We studied mitochondria from Trx2 transgenic mice to determine whether Trx2 protects against oxidant-induced MPT. All experiments were performed in isolated mitochondria. Results showed that Trx2 protected against MPT induced by exogenously added peroxide. Unexpectedly, Trx2 also protected against the MPT induced by Ca2+ in the absence of added peroxide. The results indicate that in addition to protecting against oxidative stress, Trx2 is an endogenous regulator of the MPT.

KW - Apoptosis

KW - Calcium

KW - Cell death mechanisms

KW - Necrosis

KW - Transgenic mice

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Identification of thioredoxin-2 as a regulator of the mitochondrial permeability transition

Abstract

Keywords

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