Identification of two novel functional tRNA-derived fragments induced in response to respiratory syncytial virus infection

Jiehua Zhou, Shenxuan Liu, Yu Chen, Yu Fu, Alexander J. Silver, Mark S. Hill, Inhan Lee, Yong Sun Lee, Xiaoyong Bao

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

Respiratory syncytial virus (RSV) is the leading cause of lower respiratory tract infection (LRTI) in children from infancy up to early childhood. Recently, we demonstrated that RSV infection alters cellular small non-coding RNA (sncRNA) expression, most notably the tRNA-derived RNA fragments (tRFs). However, the functions of the tRFs in virus–host interaction are largely unknown. Herein, we examined the role of three RSV-induced tRFs derived from the 5-end of mature tRNAs decoding GlyCCC, LysCTT and CysGCA (named tRF5-GlyCCC, tRF5-LysCTT and tRF5-CysGCA, respectively) in controlling RSV replication. We found that tRF5-GlyCCC and tRF5-LysCTT, but not tRF5-CysGCA, promote RSV replication, demonstrating the functional specificity of tRFs. The associated molecular mechanisms underlying the functions of tRF5-GlyCCC and tRF5LysCTT were also investigated. Regulating the expression and/or activity of these tRFs may provide new insights into preventive and therapeutic strategies for RSV infection. The study also accumulated data for future development of a tRF targeting algorithm.

Original languageEnglish (US)
Article number000852
Pages (from-to)1600-1610
Number of pages11
JournalJournal of General Virology
Volume98
Issue number7
DOIs
StatePublished - Jul 2017

Keywords

  • RSV
  • Viral replication
  • tRNA-derived RNA fragments

ASJC Scopus subject areas

  • Virology

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