Identification of Unc119 as a novel activator of Src-type tyrosine kinases

Osman Cen, Magdalena M. Gorska, Susan J. Stafford, Sanjiv Sur, Rafeul Alam

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Lyn, an Src-type tyrosine kinase, is associated with the interleukin (IL)-5 receptor in eosinophils. The mechanism of its activation is unknown. Through yeast two-hybrid screening we have cloned and characterized a new signaling molecule, Unc119, that associates with IL-5Rα and Src family tyrosine kinases. Unc119 induces the catalytic activity of these kinases through interaction with Src homology 2 and 3 domains. IL-5 stimulation of eosinophils increases Unc119 association with Lyn and induces its catalytic activity. Lyn is important for eosinophil survival. Eosinophils that are transduced with Unc119 have increased Lyn activity and demonstrate prolonged survival in the absence of IL-5. Inhibition of Unc119 down-regulates eosinophil survival. To our knowledge Unc119 is the first receptor-associated activator of Src family tyrosine kinases.

Original languageEnglish (US)
Pages (from-to)8837-8845
Number of pages9
JournalJournal of Biological Chemistry
Volume278
Issue number10
DOIs
StatePublished - Mar 7 2003

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src-Family Kinases
Eosinophils
Interleukin-5
Catalyst activity
Interleukin-5 Receptors
Interleukins
Yeast
Screening
Phosphotransferases
Chemical activation
Association reactions
Molecules
Down-Regulation
Yeasts

ASJC Scopus subject areas

  • Biochemistry

Cite this

Identification of Unc119 as a novel activator of Src-type tyrosine kinases. / Cen, Osman; Gorska, Magdalena M.; Stafford, Susan J.; Sur, Sanjiv; Alam, Rafeul.

In: Journal of Biological Chemistry, Vol. 278, No. 10, 07.03.2003, p. 8837-8845.

Research output: Contribution to journalArticle

Cen, O, Gorska, MM, Stafford, SJ, Sur, S & Alam, R 2003, 'Identification of Unc119 as a novel activator of Src-type tyrosine kinases', Journal of Biological Chemistry, vol. 278, no. 10, pp. 8837-8845. https://doi.org/10.1074/jbc.M208261200
Cen, Osman ; Gorska, Magdalena M. ; Stafford, Susan J. ; Sur, Sanjiv ; Alam, Rafeul. / Identification of Unc119 as a novel activator of Src-type tyrosine kinases. In: Journal of Biological Chemistry. 2003 ; Vol. 278, No. 10. pp. 8837-8845.
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