Identification of variables critical to reproducible delipidation of serum

Ann Marie Capriotti, Michael Laposata

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

We have developed a modified method for delipidation of serum which allows for highly reproducible preparations. Using this method, rigorous control of experimental conditions for investigation of lipid metabolism in vitro can be attained. To consistently obtain dry, white flakes of protein which readily dissolve to form delipidated serum, we have established that (a) the exact time at which the delipidated protein precipitate is rinsed with ethyl ether and (b) the thickness of the filtered protein cake, quantitated as square centimeter filter area per milliliter of serum delipidated, must be carefully controlled.

Original languageEnglish (US)
Pages (from-to)219-221
Number of pages3
JournalJournal of Tissue Culture Methods
Volume10
Issue number4
DOIs
StatePublished - Dec 1986

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Keywords

  • delipidation
  • lipids
  • tissue culture

ASJC Scopus subject areas

  • Anatomy
  • Pathology and Forensic Medicine

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