Identification of variables critical to reproducible delipidation of serum

Ann Marie Capriotti, Michael Laposata

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

We have developed a modified method for delipidation of serum which allows for highly reproducible preparations. Using this method, rigorous control of experimental conditions for investigation of lipid metabolism in vitro can be attained. To consistently obtain dry, white flakes of protein which readily dissolve to form delipidated serum, we have established that (a) the exact time at which the delipidated protein precipitate is rinsed with ethyl ether and (b) the thickness of the filtered protein cake, quantitated as square centimeter filter area per milliliter of serum delipidated, must be carefully controlled.

Original languageEnglish (US)
Pages (from-to)219-221
Number of pages3
JournalJournal of Tissue Culture Methods
Volume10
Issue number4
DOIs
StatePublished - Dec 1986
Externally publishedYes

Fingerprint

Proteins
Serum
Lipid Metabolism
Ether
Precipitates
Ethers
In Vitro Techniques

Keywords

  • delipidation
  • lipids
  • tissue culture

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Anatomy

Cite this

Identification of variables critical to reproducible delipidation of serum. / Capriotti, Ann Marie; Laposata, Michael.

In: Journal of Tissue Culture Methods, Vol. 10, No. 4, 12.1986, p. 219-221.

Research output: Contribution to journalArticle

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