Identification of viral mutants by mass spectrometry

J. Kathleen Lewis, Mohammed Bendahmane, Thomas Smith, Roger N. Beachy, Gary Siuzdak

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

A method to identify mutations of virus proteins by using protein mass mapping is described. Comparative mass mapping was applied to a structural protein of the human rhinovirus Cys1199 → Tyr mutant and to genetically engineered mutants of tobacco mosaic virus. The information generated from this approach can rapidly identify the peptide or protein containing the mutation and, in cases when nucleic acid sequencing is required, significantly narrows the region of the genome that must be sequenced. High- resolution matrix-assisted laser desorption/ionization (MALDI) mass spectrometry and tandem mass spectrometry were used to identify amino acid substitutions. This method provides valuable information for those analyzing viral variants and, in some cases, offers a rapid and accurate alternative to nucleotide sequencing.

Original languageEnglish (US)
Pages (from-to)8596-8601
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume95
Issue number15
DOIs
StatePublished - Jul 21 1998
Externally publishedYes

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Mass Spectrometry
Tandem Mass Spectrometry
Proteins
Tobacco Mosaic Virus
Rhinovirus
Mutation
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Amino Acid Substitution
Nucleic Acids
Nucleotides
Genome
Viruses
Peptides

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Identification of viral mutants by mass spectrometry. / Lewis, J. Kathleen; Bendahmane, Mohammed; Smith, Thomas; Beachy, Roger N.; Siuzdak, Gary.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, No. 15, 21.07.1998, p. 8596-8601.

Research output: Contribution to journalArticle

Lewis, J. Kathleen ; Bendahmane, Mohammed ; Smith, Thomas ; Beachy, Roger N. ; Siuzdak, Gary. / Identification of viral mutants by mass spectrometry. In: Proceedings of the National Academy of Sciences of the United States of America. 1998 ; Vol. 95, No. 15. pp. 8596-8601.
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