Identification of vitamin D-stimulated alkaline phosphatase in IEC-6 cells, a rat small intestine crypt cell line

Yow Jiun Jeng, Cheryl S. Watson, Mary L. Thomas

    Research output: Contribution to journalArticle

    17 Citations (Scopus)

    Abstract

    The IEC-6 cell line was derived from newborn rat small intestinal crypts and maintains characteristics of nontransformed crypt epithelial cells. We have reported that alkaline phosphatase (ALP) activity, a differentiation marker for intestinal epithelial cells, is regulated in IEC-6 cells by 1,25- dihydroxycholecalciferol (1,25(OH)2D3). Using reverse transcription polymerase chain reaction, we determined that the ALP in IEC-6 cells is the liver/bone/kidney ALP (LALP) isoenzyme and not the mature enterocyte form. Levamisole, a specific blocker for LALP, blocked the ALP activity of IEC-6 cells. Kinetic studies showed that 1,25(OH)2D3 increases V(m) values of ALP in IEC-6 cells while K(m) values remain the same. Northern analysis of LALP mRNA levels in IEC-6 cells indicated that 10-7 M 1,25(OH)2D3 acts by increasing alkaline phosphatase activity at the gene expression level.

    Original languageEnglish (US)
    Pages (from-to)338-343
    Number of pages6
    JournalExperimental Cell Research
    Volume212
    Issue number2
    DOIs
    StatePublished - 1994

    Fingerprint

    Vitamin D
    Small Intestine
    Alkaline Phosphatase
    Cell Line
    Kidney
    Bone and Bones
    Liver
    Epithelial Cells
    Levamisole
    Enterocytes
    Calcitriol
    Differentiation Antigens
    Isoenzymes
    Reverse Transcription
    Gene Expression
    Polymerase Chain Reaction
    Messenger RNA

    ASJC Scopus subject areas

    • Cell Biology

    Cite this

    Identification of vitamin D-stimulated alkaline phosphatase in IEC-6 cells, a rat small intestine crypt cell line. / Jeng, Yow Jiun; Watson, Cheryl S.; Thomas, Mary L.

    In: Experimental Cell Research, Vol. 212, No. 2, 1994, p. 338-343.

    Research output: Contribution to journalArticle

    Jeng, Yow Jiun ; Watson, Cheryl S. ; Thomas, Mary L. / Identification of vitamin D-stimulated alkaline phosphatase in IEC-6 cells, a rat small intestine crypt cell line. In: Experimental Cell Research. 1994 ; Vol. 212, No. 2. pp. 338-343.
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