IFCC Guideline for sampling, measuring and reporting ionized magnesium in plasma

Mohammed C. Ben Rayana, Robert W. Burnett, Arthur K. Covington, Paul D'Orazio, Niels Fogh-Andersen, Ellis Jacobs, Wolf R. Külpmann, Katsuhiko Kuwa, Lasse Larsson, Andrzej Lewenstam, Anton H J Maas, Gerhard Mager, Jerzy W. Naskalski, Anthony Okorodudu, Christoph Ritter, Andrew St John

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Analyzers with ion-selective electrodes (ISEs) for ionized magnesium (iMg) should yield comparable and unbiased results for iMg. This IFCC guideline on sampling, measuring and reporting iMg in plasma provides a prerequisite to achieve this goal [in this document, "plasma" refers to circulating plasma and the forms in which it is sampled, namely the plasma phase of anticoagulated whole blood (or "blood"), plasma separated from blood cells, or serum]. The guideline recommends measuring and reporting ionized magnesium as a substance concentration relative to the substance concentration of magnesium in primary aqueous calibrants with magnesium, sodium, and calcium chloride of physiological ionic strength. The recommended name is "the concentration of ionized magnesium in plasma". Based on this guideline, results will be approximately 3% higher than the true substance concentration and 4% lower than the true molality in plasma. Calcium ions interfere with all current magnesium ion-selective electrodes (Mg-ISEs), and thus it is necessary to determine both ions simultaneously in each sample and correct the result for Ca2+ interference. Binding of Mg in plasma is pH-dependent. Therefore, pH should be measured simultaneously with iMg to allow adjustment of the result to pH 7.4. The concentration of iMg in plasma may be physiologically and clinically more relevant than the concentration of total magnesium. Furthermore, blood-gas analyzers or instruments for point-of-care testing are able to measure plasma iMg using whole blood (with intact blood cells) as the sample, minimizing turn-around time compared to serum and plasma, which require removal of blood cells.

Original languageEnglish (US)
Pages (from-to)21-26
Number of pages6
JournalClinical Chemistry and Laboratory Medicine
Volume46
Issue number1
DOIs
StatePublished - Jan 1 2008
Externally publishedYes

Fingerprint

Magnesium
Guidelines
Sampling
Plasmas
Blood
Ion-Selective Electrodes
Blood Cells
Cells
Ions
Calcium Chloride
Turnaround time
Magnesium Chloride
Ionic strength
Serum
Sodium Chloride
Osmolar Concentration
Names
Gases
Calcium
Testing

Keywords

  • Erythrocyte effect
  • Heparin
  • Influence factors
  • Interference
  • Ion-selective electrode
  • Ionized calcium
  • Ionized magnesium
  • Lipophilic anions
  • Liquid junction potential
  • pH
  • Protein
  • Sampling
  • Silicone
  • Sodium

ASJC Scopus subject areas

  • Clinical Biochemistry

Cite this

Ben Rayana, M. C., Burnett, R. W., Covington, A. K., D'Orazio, P., Fogh-Andersen, N., Jacobs, E., ... St John, A. (2008). IFCC Guideline for sampling, measuring and reporting ionized magnesium in plasma. Clinical Chemistry and Laboratory Medicine, 46(1), 21-26. https://doi.org/10.1515/CCLM.2008.001

IFCC Guideline for sampling, measuring and reporting ionized magnesium in plasma. / Ben Rayana, Mohammed C.; Burnett, Robert W.; Covington, Arthur K.; D'Orazio, Paul; Fogh-Andersen, Niels; Jacobs, Ellis; Külpmann, Wolf R.; Kuwa, Katsuhiko; Larsson, Lasse; Lewenstam, Andrzej; Maas, Anton H J; Mager, Gerhard; Naskalski, Jerzy W.; Okorodudu, Anthony; Ritter, Christoph; St John, Andrew.

In: Clinical Chemistry and Laboratory Medicine, Vol. 46, No. 1, 01.01.2008, p. 21-26.

Research output: Contribution to journalArticle

Ben Rayana, MC, Burnett, RW, Covington, AK, D'Orazio, P, Fogh-Andersen, N, Jacobs, E, Külpmann, WR, Kuwa, K, Larsson, L, Lewenstam, A, Maas, AHJ, Mager, G, Naskalski, JW, Okorodudu, A, Ritter, C & St John, A 2008, 'IFCC Guideline for sampling, measuring and reporting ionized magnesium in plasma', Clinical Chemistry and Laboratory Medicine, vol. 46, no. 1, pp. 21-26. https://doi.org/10.1515/CCLM.2008.001
Ben Rayana MC, Burnett RW, Covington AK, D'Orazio P, Fogh-Andersen N, Jacobs E et al. IFCC Guideline for sampling, measuring and reporting ionized magnesium in plasma. Clinical Chemistry and Laboratory Medicine. 2008 Jan 1;46(1):21-26. https://doi.org/10.1515/CCLM.2008.001
Ben Rayana, Mohammed C. ; Burnett, Robert W. ; Covington, Arthur K. ; D'Orazio, Paul ; Fogh-Andersen, Niels ; Jacobs, Ellis ; Külpmann, Wolf R. ; Kuwa, Katsuhiko ; Larsson, Lasse ; Lewenstam, Andrzej ; Maas, Anton H J ; Mager, Gerhard ; Naskalski, Jerzy W. ; Okorodudu, Anthony ; Ritter, Christoph ; St John, Andrew. / IFCC Guideline for sampling, measuring and reporting ionized magnesium in plasma. In: Clinical Chemistry and Laboratory Medicine. 2008 ; Vol. 46, No. 1. pp. 21-26.
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