IFN-γ Decreases Translocation and Improves Survival Following Transfusion and Thermal Injury

Roberto Gennari, J. Wesley Alexander, Tonyia Eaves-Pyles

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

The effects of recombinant murine interferon-γ (rmIFN-γ) on survival and host defense were studied during gut-derived sepsis that included transfusion-induced immunosuppression. Balb/c mice (n = 153) were transfused with allogeneic blood and then treated with different doses of rmIFN-γ: 10, 100, 1000, 10,000 U, or sterile saline as control once daily for 3 days. Five days after transfusion they were gavaged with 1010Escherichia coli and given a 20% TBSA burn injury. Survival was significantly higher in groups receiving 10 U compared to control and the group receiving 10,000 U (P < 0.0001 and P - 0.02, respectively). Groups receiving 100 or 1000 U also showed an improvement of survival compared to nontreated control animals (P = 0.02). The effect of rmIFN-γ on the degree of translocation and the host's ability to kill translocated organisms was also investigated. Mice were treated as described above, except they were gavaged with 111In oxine-labeled E. coli and then subjected to a 20% TBSA burn. Mesenteric lymph nodes (MLN), liver, and spleen were harvested aseptically. Less translocation to the liver was observed compared to the nontreated group (P = 0.002) to the MLNs and spleen of the group treated with 100 U rmIFN-γ compared to controls and the group treated with 10 U (P < 0.005). Animals receiving 1000 U showed fewer bacteria in the liver and spleen compared to the control group (P < 0.005). Moreover, the ability to kill translocated organisms was significantly enhanced in the liver of the animals receiving 10 or 100 U rmIFN-γ compared to all other groups (P < 0.0001). In conclusion, the study suggests that treatment with 10 and 100 U of rmIFN-γ positively affects the outcome in gut-derived sepsis, mainly by enhancing killing of translocated bacteria. The beneficial effect in animals receiving 100 or 1000 U was related to an improved gut barrier function.

Original languageEnglish (US)
Pages (from-to)530-536
Number of pages7
JournalJournal of Surgical Research
Volume56
Issue number6
DOIs
StatePublished - Jun 1994
Externally publishedYes

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Interferons
Hot Temperature
Wounds and Injuries
Spleen
Liver
Control Groups
Sepsis
Bacteria
Immunosuppression
Lymph Nodes
Escherichia coli

ASJC Scopus subject areas

  • Surgery

Cite this

IFN-γ Decreases Translocation and Improves Survival Following Transfusion and Thermal Injury. / Gennari, Roberto; Alexander, J. Wesley; Eaves-Pyles, Tonyia.

In: Journal of Surgical Research, Vol. 56, No. 6, 06.1994, p. 530-536.

Research output: Contribution to journalArticle

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abstract = "The effects of recombinant murine interferon-γ (rmIFN-γ) on survival and host defense were studied during gut-derived sepsis that included transfusion-induced immunosuppression. Balb/c mice (n = 153) were transfused with allogeneic blood and then treated with different doses of rmIFN-γ: 10, 100, 1000, 10,000 U, or sterile saline as control once daily for 3 days. Five days after transfusion they were gavaged with 1010Escherichia coli and given a 20{\%} TBSA burn injury. Survival was significantly higher in groups receiving 10 U compared to control and the group receiving 10,000 U (P < 0.0001 and P - 0.02, respectively). Groups receiving 100 or 1000 U also showed an improvement of survival compared to nontreated control animals (P = 0.02). The effect of rmIFN-γ on the degree of translocation and the host's ability to kill translocated organisms was also investigated. Mice were treated as described above, except they were gavaged with 111In oxine-labeled E. coli and then subjected to a 20{\%} TBSA burn. Mesenteric lymph nodes (MLN), liver, and spleen were harvested aseptically. Less translocation to the liver was observed compared to the nontreated group (P = 0.002) to the MLNs and spleen of the group treated with 100 U rmIFN-γ compared to controls and the group treated with 10 U (P < 0.005). Animals receiving 1000 U showed fewer bacteria in the liver and spleen compared to the control group (P < 0.005). Moreover, the ability to kill translocated organisms was significantly enhanced in the liver of the animals receiving 10 or 100 U rmIFN-γ compared to all other groups (P < 0.0001). In conclusion, the study suggests that treatment with 10 and 100 U of rmIFN-γ positively affects the outcome in gut-derived sepsis, mainly by enhancing killing of translocated bacteria. The beneficial effect in animals receiving 100 or 1000 U was related to an improved gut barrier function.",
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