IgE-independent mast cell activation augments contractility of nonpregnant and pregnant guinea pig myometrium

Egle Bytautiene, Yuri P. Vedernikov, George Saade, Roberto Romero, Robert E. Garfield

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Background: We have previously shown that in sensitized guinea pigs premature labor can be induced by a type I hypersensitivity reaction. We further hypothesize that premature labor occurs due to increased uterine contractility caused by activation of mast cells and possibly eosinophils, and collective release of their mediators. The objective of this study was to test the hypothesis that IgE-independent mast cell degranulation could increase uterine contractility. Methods: Longitudinal uterine strips from nonpregnant and pregnant guinea pigs were incubated in organ chambers with vehicle, histamine H1, serotonin 5-HT2/5-HT1C, thromboxane A 2, leukotriene D4 receptor antagonists, mast cell stabilizer, and cyclooxygenase or lipoxygenase inhibitors. Then, supernatant, obtained after activation of a mast cell line (MC/9) with compound 48/80, culture medium, or compound 48/80 alone were added. Cumulative concentration-response curves to histamine and serotonin were also obtained. Results: The supernatant and compound 48/80 significantly increased contractility of uterine strips. A mast cell stabilizer considerably reduced the effect of compound 48/80. Other substances attenuated uterine contractile responses to supernatant and compound 48/80, and responses varied depending on the pregnancy period. Histamine and serotonin increased contractility of uterine strips, and uterine sensitivity to these agents were dependent on gestational age. Conclusions: In summary, mast cells increase uterine contractility through multiple mediators, and uterine responses to these mediators are dependent on gestational age. We postulate that the simultaneous release of these mast cell/eosinophil mediators in the uterus could be a stimulus to trigger and/or maintain myometrial contractions during preterm and term labor.

Original languageEnglish (US)
Pages (from-to)140-146
Number of pages7
JournalInternational Archives of Allergy and Immunology
Volume147
Issue number2
DOIs
StatePublished - Sep 2008

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Myometrium
Mast Cells
p-Methoxy-N-methylphenethylamine
Immunoglobulin E
Guinea Pigs
Premature Obstetric Labor
Histamine
Serotonin
Eosinophils
Gestational Age
Leukotriene Antagonists
Cell Degranulation
Immediate Hypersensitivity
Lipoxygenase Inhibitors
Uterine Contraction
Cyclooxygenase Inhibitors
Thromboxanes
varespladib methyl
Uterus
Culture Media

Keywords

  • Cell line MC/9
  • Compound 48/80
  • Hypersensitivity reaction
  • Mast cells
  • Myometrial contraction
  • Premature labor
  • Type I hypersensitivity
  • Uterine contractility

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

IgE-independent mast cell activation augments contractility of nonpregnant and pregnant guinea pig myometrium. / Bytautiene, Egle; Vedernikov, Yuri P.; Saade, George; Romero, Roberto; Garfield, Robert E.

In: International Archives of Allergy and Immunology, Vol. 147, No. 2, 09.2008, p. 140-146.

Research output: Contribution to journalArticle

Bytautiene, Egle ; Vedernikov, Yuri P. ; Saade, George ; Romero, Roberto ; Garfield, Robert E. / IgE-independent mast cell activation augments contractility of nonpregnant and pregnant guinea pig myometrium. In: International Archives of Allergy and Immunology. 2008 ; Vol. 147, No. 2. pp. 140-146.
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