IGF-I gene transfer effects on inflammatory elements present after thermal trauma

Mohan R K Dasu, David Herndon, Olivera Nesic, J. Regino Perez-Polo

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Major thermal injury results in severe prolonged responses with three components: a hypermetabolic response, inflammatory responses, and endogenous wound-healing processes. We showed that use of liposome-mediated gene transfer of the insulin-like growth factor I (IGF-I) reduces burn-induced inflammatory responses and enhances wound healing. In the present study, we found transient increased levels of IGF-I protein in rats exposed to thermal trauma via liposomal gene transfer in an effort to define the transcriptional events that occur after IGF-I delivery at the site of injury. The beneficial effects of IGF-I gene transfer act partly via amelioration of burn-induced inflammatory responses that mediate cell death through caspase-3 activity and Bax expression. IGF-I gene transfer induces selective stimulation of activation protein-1 DNA-binding activity and activation of antiapoptotic, but not inflammatory, NF-κB transcription factors. Data were consistent with our hypothesis that the beneficial effects of IGF-I gene transfer on burned rats act in part via activation protein-1 and NF-κB transcriptional regulation and the concordance between the results obtained with antiapoptotic, as opposed to the proapoptotic, sequences as well as the corresponding changes in measures of cell death via Bax and caspase-3 mechanisms.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Regulatory Integrative and Comparative Physiology
Volume285
Issue number4 54-4
StatePublished - Oct 1 2003

Fingerprint

Insulin-Like Growth Factor I
Hot Temperature
Wounds and Injuries
Genes
Burns
Caspase 3
Wound Healing
Cell Death
Neurofibromin 1
DNA-Binding Proteins
Liposomes
Transcription Factors
Proteins

Keywords

  • Activation protein-1
  • Bax
  • Caspase-3
  • Nuclear factor-κB
  • Thermal injury

ASJC Scopus subject areas

  • Physiology

Cite this

IGF-I gene transfer effects on inflammatory elements present after thermal trauma. / Dasu, Mohan R K; Herndon, David; Nesic, Olivera; Perez-Polo, J. Regino.

In: American Journal of Physiology - Regulatory Integrative and Comparative Physiology, Vol. 285, No. 4 54-4, 01.10.2003.

Research output: Contribution to journalArticle

Dasu, Mohan R K ; Herndon, David ; Nesic, Olivera ; Perez-Polo, J. Regino. / IGF-I gene transfer effects on inflammatory elements present after thermal trauma. In: American Journal of Physiology - Regulatory Integrative and Comparative Physiology. 2003 ; Vol. 285, No. 4 54-4.
@article{e75e3d77b09f4fd6a444db3c5e5d4767,
title = "IGF-I gene transfer effects on inflammatory elements present after thermal trauma",
abstract = "Major thermal injury results in severe prolonged responses with three components: a hypermetabolic response, inflammatory responses, and endogenous wound-healing processes. We showed that use of liposome-mediated gene transfer of the insulin-like growth factor I (IGF-I) reduces burn-induced inflammatory responses and enhances wound healing. In the present study, we found transient increased levels of IGF-I protein in rats exposed to thermal trauma via liposomal gene transfer in an effort to define the transcriptional events that occur after IGF-I delivery at the site of injury. The beneficial effects of IGF-I gene transfer act partly via amelioration of burn-induced inflammatory responses that mediate cell death through caspase-3 activity and Bax expression. IGF-I gene transfer induces selective stimulation of activation protein-1 DNA-binding activity and activation of antiapoptotic, but not inflammatory, NF-κB transcription factors. Data were consistent with our hypothesis that the beneficial effects of IGF-I gene transfer on burned rats act in part via activation protein-1 and NF-κB transcriptional regulation and the concordance between the results obtained with antiapoptotic, as opposed to the proapoptotic, sequences as well as the corresponding changes in measures of cell death via Bax and caspase-3 mechanisms.",
keywords = "Activation protein-1, Bax, Caspase-3, Nuclear factor-κB, Thermal injury",
author = "Dasu, {Mohan R K} and David Herndon and Olivera Nesic and Perez-Polo, {J. Regino}",
year = "2003",
month = "10",
day = "1",
language = "English (US)",
volume = "285",
journal = "American Journal of Physiology - Endocrinology and Metabolism",
issn = "0193-1849",
publisher = "American Physiological Society",
number = "4 54-4",

}

TY - JOUR

T1 - IGF-I gene transfer effects on inflammatory elements present after thermal trauma

AU - Dasu, Mohan R K

AU - Herndon, David

AU - Nesic, Olivera

AU - Perez-Polo, J. Regino

PY - 2003/10/1

Y1 - 2003/10/1

N2 - Major thermal injury results in severe prolonged responses with three components: a hypermetabolic response, inflammatory responses, and endogenous wound-healing processes. We showed that use of liposome-mediated gene transfer of the insulin-like growth factor I (IGF-I) reduces burn-induced inflammatory responses and enhances wound healing. In the present study, we found transient increased levels of IGF-I protein in rats exposed to thermal trauma via liposomal gene transfer in an effort to define the transcriptional events that occur after IGF-I delivery at the site of injury. The beneficial effects of IGF-I gene transfer act partly via amelioration of burn-induced inflammatory responses that mediate cell death through caspase-3 activity and Bax expression. IGF-I gene transfer induces selective stimulation of activation protein-1 DNA-binding activity and activation of antiapoptotic, but not inflammatory, NF-κB transcription factors. Data were consistent with our hypothesis that the beneficial effects of IGF-I gene transfer on burned rats act in part via activation protein-1 and NF-κB transcriptional regulation and the concordance between the results obtained with antiapoptotic, as opposed to the proapoptotic, sequences as well as the corresponding changes in measures of cell death via Bax and caspase-3 mechanisms.

AB - Major thermal injury results in severe prolonged responses with three components: a hypermetabolic response, inflammatory responses, and endogenous wound-healing processes. We showed that use of liposome-mediated gene transfer of the insulin-like growth factor I (IGF-I) reduces burn-induced inflammatory responses and enhances wound healing. In the present study, we found transient increased levels of IGF-I protein in rats exposed to thermal trauma via liposomal gene transfer in an effort to define the transcriptional events that occur after IGF-I delivery at the site of injury. The beneficial effects of IGF-I gene transfer act partly via amelioration of burn-induced inflammatory responses that mediate cell death through caspase-3 activity and Bax expression. IGF-I gene transfer induces selective stimulation of activation protein-1 DNA-binding activity and activation of antiapoptotic, but not inflammatory, NF-κB transcription factors. Data were consistent with our hypothesis that the beneficial effects of IGF-I gene transfer on burned rats act in part via activation protein-1 and NF-κB transcriptional regulation and the concordance between the results obtained with antiapoptotic, as opposed to the proapoptotic, sequences as well as the corresponding changes in measures of cell death via Bax and caspase-3 mechanisms.

KW - Activation protein-1

KW - Bax

KW - Caspase-3

KW - Nuclear factor-κB

KW - Thermal injury

UR - http://www.scopus.com/inward/record.url?scp=0141522498&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0141522498&partnerID=8YFLogxK

M3 - Article

C2 - 12805089

AN - SCOPUS:0141522498

VL - 285

JO - American Journal of Physiology - Endocrinology and Metabolism

JF - American Journal of Physiology - Endocrinology and Metabolism

SN - 0193-1849

IS - 4 54-4

ER -