IKKε modulates RSV-induced NF-?B-dependent gene transcription

Xiaoyong Bao, Hemalatha Indukuri, Tianshuang Liu, Sui Ling Liao, Bing Tian, Allan R. Brasier, Roberto Garofalo, Antonella Casola

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Respiratory syncytial virus (RSV), a negative-strand RNA virus, is the most common cause of epidemic respiratory disease in infants and young children. RSV infection of airway epithelial cells induces the expression of immune/inflammatory genes through the activation of a subset of transcription factors, including Nuclear Factor-?B (NF-?B). In this study we have investigated the role of the non canonical I?B kinase (IKK)ε in modulating RSV-induced NF-?B activation. Our results show that inhibition of IKK? activation results in significant impairment of viral-induced NF-?B-dependent gene expression, through a reduction in NF-?B transcriptional activity, without changes in nuclear translocation or DNA-binding activity. Absence of IKK? results in a significant decrease of RSV-induced NF-?B phosphorylation on serine 536, a post-translational modification important for RSV-induced NF-?B-dependent gene expression, known to regulate NF-?B transcriptional activity without affecting nuclear translocation. This study identifies a novel mechanism by which IKK? regulates viral-induced cellular signaling.

Original languageEnglish (US)
Pages (from-to)224-231
Number of pages8
JournalVirology
Volume408
Issue number2
DOIs
StatePublished - Dec 20 2010

Fingerprint

Respiratory Syncytial Viruses
Genes
Gene Expression
Respiratory Syncytial Virus Infections
RNA Viruses
Post Translational Protein Processing
Serine
Transcriptional Activation
Transcription Factors
Phosphotransferases
Epithelial Cells
Phosphorylation
DNA

Keywords

  • Airway epithelial cells
  • Inflammation
  • NF-?B
  • RSV

ASJC Scopus subject areas

  • Virology

Cite this

IKKε modulates RSV-induced NF-?B-dependent gene transcription. / Bao, Xiaoyong; Indukuri, Hemalatha; Liu, Tianshuang; Liao, Sui Ling; Tian, Bing; Brasier, Allan R.; Garofalo, Roberto; Casola, Antonella.

In: Virology, Vol. 408, No. 2, 20.12.2010, p. 224-231.

Research output: Contribution to journalArticle

Bao, Xiaoyong ; Indukuri, Hemalatha ; Liu, Tianshuang ; Liao, Sui Ling ; Tian, Bing ; Brasier, Allan R. ; Garofalo, Roberto ; Casola, Antonella. / IKKε modulates RSV-induced NF-?B-dependent gene transcription. In: Virology. 2010 ; Vol. 408, No. 2. pp. 224-231.
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