IL-1β induction of NF-κB activation in human intestinal epithelial cells is independent of oxyradical signaling

Alexander A. Parikh, M. Ryan Moon, Timothy A. Pritts, Josef E. Fischer, Csaba Szabo, Per Olof Hasselgren, Andrew L. Salzman

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

IL-1β stimulation of cultured epithelial cells induces the degradation of IκBα and the consequent nuclear translocation of NF-λB, a critical proinflammatory transcription factor in the mucosal host immune response. The role of reactive oxygen intermediates, serine protease activity, and tyrosine kinase activity in the activation of NF-κB is weakly conserved across various cell lineages and has not been defined in human enterocytes, a major target of oxidant stress in sepsis, thermal injury, and hemorrhagic shock. We report here that in Caco-2BBe cells, a transformed human colon cancer cell line with features of small intestinal epithelial cells in culture, exposure to oxidant stress (hydrogen peroxide 1-10 mM) did not induce NF-κB activation. Similarly, scavenging of free radicals and oxidants by pyrrolidine dithiocarbamate and dimethyl sulfoxide did not block IL-1β-induced IκBα degradation and NF-κB activation. Genistein, a non-specific tyrosine kinase inhibitor, also had no effect on IL-1β-mediated effects on NF-κB. Serine protease inhibition by tosyl-lysine-chloromethylketone and tosyl-phenylalanine-chloromethylketone inhibited IκBα degradation and NF-κB activation stimulated by IL-1β. Our data highlight the strong divergence between epithelial and mononuclear cells in the signal transduction pathways relating IL-1β stimulation and NF-κB nuclear translocation.

Original languageEnglish (US)
Pages (from-to)8-13
Number of pages6
JournalShock
Volume13
Issue number1
StatePublished - Jan 2000
Externally publishedYes

Fingerprint

Interleukin-1
Epithelial Cells
Oxidants
Serine Proteases
Protein-Tyrosine Kinases
Mucosal Immunity
Hemorrhagic Shock
Enterocytes
Genistein
Cell Lineage
Dimethyl Sulfoxide
Phenylalanine
Colonic Neoplasms
Hydrogen Peroxide
Lysine
Free Radicals
Cultured Cells
Signal Transduction
Sepsis
Transcription Factors

Keywords

  • Cytokines
  • Inflammation
  • Mucosa
  • Sepsis
  • Transcription factor

ASJC Scopus subject areas

  • Physiology
  • Critical Care and Intensive Care Medicine

Cite this

Parikh, A. A., Moon, M. R., Pritts, T. A., Fischer, J. E., Szabo, C., Hasselgren, P. O., & Salzman, A. L. (2000). IL-1β induction of NF-κB activation in human intestinal epithelial cells is independent of oxyradical signaling. Shock, 13(1), 8-13.

IL-1β induction of NF-κB activation in human intestinal epithelial cells is independent of oxyradical signaling. / Parikh, Alexander A.; Moon, M. Ryan; Pritts, Timothy A.; Fischer, Josef E.; Szabo, Csaba; Hasselgren, Per Olof; Salzman, Andrew L.

In: Shock, Vol. 13, No. 1, 01.2000, p. 8-13.

Research output: Contribution to journalArticle

Parikh, AA, Moon, MR, Pritts, TA, Fischer, JE, Szabo, C, Hasselgren, PO & Salzman, AL 2000, 'IL-1β induction of NF-κB activation in human intestinal epithelial cells is independent of oxyradical signaling', Shock, vol. 13, no. 1, pp. 8-13.
Parikh AA, Moon MR, Pritts TA, Fischer JE, Szabo C, Hasselgren PO et al. IL-1β induction of NF-κB activation in human intestinal epithelial cells is independent of oxyradical signaling. Shock. 2000 Jan;13(1):8-13.
Parikh, Alexander A. ; Moon, M. Ryan ; Pritts, Timothy A. ; Fischer, Josef E. ; Szabo, Csaba ; Hasselgren, Per Olof ; Salzman, Andrew L. / IL-1β induction of NF-κB activation in human intestinal epithelial cells is independent of oxyradical signaling. In: Shock. 2000 ; Vol. 13, No. 1. pp. 8-13.
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