IL-1 receptor antagonist prevents apoptosis and caspase-3 activation after spinal cord injury

O. Nesic, G. Y. Xu, D. McAdoo, K. W. High, C. Hulsebosch, R. Perez-Polo

Research output: Contribution to journalArticle

129 Citations (Scopus)

Abstract

One of the consequences of cytokine-orchestrated inflammation after CNS trauma is apoptosis. Our hypothesis is that cell death in the spinal cord after injury results in part from increased synthesis and release of IL-1β. Using a ribonuclease protection assay, we demonstrated that there is increased transient expression of IL-1β mRNA and, by using IL-1β protein ELISA assay, that there are increased IL-1β protein levels in the contused rat spinal cord, initially localized to the impact region of the spinal cord (segment T8). Using an ELISA cell death assay, we showed that there is apoptosis in the spinal cord 72 h after injury, a finding that was confirmed by measuring caspase-3 activity, which also significantly increased at the site of injury 72 h after trauma. Treatment of the contused spinal cord at the site of injury with the IL-1 receptor antagonist (rmIL-1ra, 750 ng/mL) for 72 h using an osmotic minipump completely abolished the increases in contusion-induced apoptosis and caspase-3 activity.

Original languageEnglish (US)
Pages (from-to)947-956
Number of pages10
JournalJournal of Neurotrauma
Volume18
Issue number9
StatePublished - 2001
Externally publishedYes

Fingerprint

Interleukin-1 Receptors
Spinal Cord Injuries
Caspase 3
Interleukin-1
Apoptosis
Contusions
Spinal Cord
Wounds and Injuries
Cell Death
Enzyme-Linked Immunosorbent Assay
Ribonucleases
Proteins
Cytokines
Inflammation
Messenger RNA

Keywords

  • 11-1β
  • Apoptosis
  • Caspase-3
  • IL-1 receptor antagonist
  • Spinal cord injury

ASJC Scopus subject areas

  • Clinical Neurology
  • Neuroscience(all)

Cite this

Nesic, O., Xu, G. Y., McAdoo, D., High, K. W., Hulsebosch, C., & Perez-Polo, R. (2001). IL-1 receptor antagonist prevents apoptosis and caspase-3 activation after spinal cord injury. Journal of Neurotrauma, 18(9), 947-956.

IL-1 receptor antagonist prevents apoptosis and caspase-3 activation after spinal cord injury. / Nesic, O.; Xu, G. Y.; McAdoo, D.; High, K. W.; Hulsebosch, C.; Perez-Polo, R.

In: Journal of Neurotrauma, Vol. 18, No. 9, 2001, p. 947-956.

Research output: Contribution to journalArticle

Nesic, O, Xu, GY, McAdoo, D, High, KW, Hulsebosch, C & Perez-Polo, R 2001, 'IL-1 receptor antagonist prevents apoptosis and caspase-3 activation after spinal cord injury', Journal of Neurotrauma, vol. 18, no. 9, pp. 947-956.
Nesic O, Xu GY, McAdoo D, High KW, Hulsebosch C, Perez-Polo R. IL-1 receptor antagonist prevents apoptosis and caspase-3 activation after spinal cord injury. Journal of Neurotrauma. 2001;18(9):947-956.
Nesic, O. ; Xu, G. Y. ; McAdoo, D. ; High, K. W. ; Hulsebosch, C. ; Perez-Polo, R. / IL-1 receptor antagonist prevents apoptosis and caspase-3 activation after spinal cord injury. In: Journal of Neurotrauma. 2001 ; Vol. 18, No. 9. pp. 947-956.
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