IL-1 stimulates intestinal myofibroblast COX gene expression and augments activation of Cl- secretion in T84 cells

Thomas A. Hinterleitner, Jamal I. Saada, Helen M. Berschneider, Don W. Powell, John D. Valentich

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

Because interleukin-1 (IL-1) is an important mediator in the inflamed intestine, its effects on enterocyte-subepithelial myofibroblast (SEMF) interaction were investigated in vitro. Acutely juxtaposing T84 cells with 18Co or P2JF SEMF preincubated with IL-1α significantly enhanced T84 short- circuit current (I(sc)) responsiveness to secretagogues in comparison to SEMF not activated by IL-1α. The sensitivity of T84 cell I(sc) to Ca2+- dependent, but not adenosine 3',5'-cyclic monophosphate-dependent, secretagogues was augmented by IL-1α-treated SEMF. These effects of IL-1α are directly correlated with SEMF prostaglandin E2 (PGE2) production. Both IL-1α augmentation of Cl- secretagogue responsiveness and PGE2 formation were inhibited by IL-1 receptor antagonist. Within 5 h, IL-1α stimulated a 10-fold increase in cyclooxygenase (COX)-2 steady-state mRNA levels in 18Co cells. In contrast, COX-1 message levels increased more slowly to two- to threefold above control levels after 24 h incubation. These results demonstrate that the proinflammatory cytokine IL-1α accentuates intestinal SEMF augmentation of enterocyte responsiveness to Ca2+-dependent Cl- secretagogues. PGE2 is an important mediator of SEMF-enterocyte interaction. The effects of IL-1α on SEMF PGE2 productions are, at least in part, due to stimulation of COX gene expression.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Cell Physiology
Volume271
Issue number4 40-4
StatePublished - Oct 1996

Fingerprint

Myofibroblasts
Prostaglandin-Endoperoxide Synthases
Interleukin-1
Gene expression
Chemical activation
Gene Expression
Dinoprostone
Enterocytes
Cyclooxygenase 1
Interleukin-1 Receptors
Level control
Cyclooxygenase 2
Short circuit currents
Cyclic AMP
Intestines
Cytokines
Messenger RNA

Keywords

  • cyclooxygenase
  • interleukin-1
  • intestines
  • prostaglandins

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Physiology
  • Physiology (medical)

Cite this

Hinterleitner, T. A., Saada, J. I., Berschneider, H. M., Powell, D. W., & Valentich, J. D. (1996). IL-1 stimulates intestinal myofibroblast COX gene expression and augments activation of Cl- secretion in T84 cells. American Journal of Physiology - Cell Physiology, 271(4 40-4).

IL-1 stimulates intestinal myofibroblast COX gene expression and augments activation of Cl- secretion in T84 cells. / Hinterleitner, Thomas A.; Saada, Jamal I.; Berschneider, Helen M.; Powell, Don W.; Valentich, John D.

In: American Journal of Physiology - Cell Physiology, Vol. 271, No. 4 40-4, 10.1996.

Research output: Contribution to journalArticle

Hinterleitner, TA, Saada, JI, Berschneider, HM, Powell, DW & Valentich, JD 1996, 'IL-1 stimulates intestinal myofibroblast COX gene expression and augments activation of Cl- secretion in T84 cells', American Journal of Physiology - Cell Physiology, vol. 271, no. 4 40-4.
Hinterleitner, Thomas A. ; Saada, Jamal I. ; Berschneider, Helen M. ; Powell, Don W. ; Valentich, John D. / IL-1 stimulates intestinal myofibroblast COX gene expression and augments activation of Cl- secretion in T84 cells. In: American Journal of Physiology - Cell Physiology. 1996 ; Vol. 271, No. 4 40-4.
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