IL-5 is the predominant eosinophil-active cytokine in the antigen-induced pulmonary late-phase reaction

T. Ohnishi, H. Kita, D. Weiler, Sanjiv Sur, J. B. Sedgwick, William Calhoun, W. W. Busse, J. S. Abrams, G. J. Gleich

Research output: Contribution to journalArticle

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Abstract

The mechanism of airway eosinophilia during antigen-induced inflammation was investigated by measurement of eosinophil-active cytokines utilizing an eosinophil survival assay. In the first study, 4 patients with allergic rhinitis underwent segmental bronchoprovocation (SBP) with low, medium, and high doses of ragweed extract instilled into different bronchial subsegments; bronchoalveolar lavage (BAL) fluids were collected from each segment 12 min and 48 h after challenge. Eosinophil granule proteins and eosinophil survival activity were significantly elevated in the 48-h (late-phase) BAL fluids from these segments. Correlations were observed between the concentrations of eosinophil granule proteins and eosinophil survival activity (r(s) = 0.717 to 0.880, p < 0.001) in BAL fluids. Eosinophil survival activity was completely neutralized by anti-IL-5 monoclonal antibody in five of the seven 48-h samples tested representing three of the 4 patients. In the two remaining samples, eosinophil survival activity was only partially neutralized by either anti-IL-5 antibody or anti-granulocyte-macrophage colony-stimulating factor (GM-CSF) but was completely neutralized by anti-IL-5 and anti-GM-CSF in combination. Subsequently, in the second study, 10 patients with allergic rhinitis were challenged by SBP with ragweed extract. Eosinophil survival activity was significantly elevated in the 48-h BAL fluids; this activity was partially neutralized by anti-IL-5 antibody about (48%) and completely neutralized by the combination of anti-IL-5 and anti-GM-CSF antibodies. These findings suggest that the eosinophil survival activity in the late inflammatory lesions following SBP with allergen is mainly associated with IL-5, with small contributions from GM-CSF. Thus, IL-5 is the predominant eosinophil-active cytokine present in BAL fluids during allergen-induced late-phase inflammation and may play a key role in the pathophysiology of allergen-induced, eosinophil-predominant airway inflammation.

Original languageEnglish (US)
Pages (from-to)901-907
Number of pages7
JournalAmerican Review of Respiratory Disease
Volume147
Issue number4
StatePublished - 1993
Externally publishedYes

Fingerprint

Interleukin-5
Eosinophils
Cytokines
Antigens
Lung
Bronchoalveolar Lavage Fluid
Granulocyte-Macrophage Colony-Stimulating Factor
Eosinophil Granule Proteins
Allergens
Ambrosia
Inflammation
Antibodies
Eosinophilia
Anti-Idiotypic Antibodies
Monoclonal Antibodies

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine

Cite this

Ohnishi, T., Kita, H., Weiler, D., Sur, S., Sedgwick, J. B., Calhoun, W., ... Gleich, G. J. (1993). IL-5 is the predominant eosinophil-active cytokine in the antigen-induced pulmonary late-phase reaction. American Review of Respiratory Disease, 147(4), 901-907.

IL-5 is the predominant eosinophil-active cytokine in the antigen-induced pulmonary late-phase reaction. / Ohnishi, T.; Kita, H.; Weiler, D.; Sur, Sanjiv; Sedgwick, J. B.; Calhoun, William; Busse, W. W.; Abrams, J. S.; Gleich, G. J.

In: American Review of Respiratory Disease, Vol. 147, No. 4, 1993, p. 901-907.

Research output: Contribution to journalArticle

Ohnishi, T, Kita, H, Weiler, D, Sur, S, Sedgwick, JB, Calhoun, W, Busse, WW, Abrams, JS & Gleich, GJ 1993, 'IL-5 is the predominant eosinophil-active cytokine in the antigen-induced pulmonary late-phase reaction', American Review of Respiratory Disease, vol. 147, no. 4, pp. 901-907.
Ohnishi, T. ; Kita, H. ; Weiler, D. ; Sur, Sanjiv ; Sedgwick, J. B. ; Calhoun, William ; Busse, W. W. ; Abrams, J. S. ; Gleich, G. J. / IL-5 is the predominant eosinophil-active cytokine in the antigen-induced pulmonary late-phase reaction. In: American Review of Respiratory Disease. 1993 ; Vol. 147, No. 4. pp. 901-907.
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abstract = "The mechanism of airway eosinophilia during antigen-induced inflammation was investigated by measurement of eosinophil-active cytokines utilizing an eosinophil survival assay. In the first study, 4 patients with allergic rhinitis underwent segmental bronchoprovocation (SBP) with low, medium, and high doses of ragweed extract instilled into different bronchial subsegments; bronchoalveolar lavage (BAL) fluids were collected from each segment 12 min and 48 h after challenge. Eosinophil granule proteins and eosinophil survival activity were significantly elevated in the 48-h (late-phase) BAL fluids from these segments. Correlations were observed between the concentrations of eosinophil granule proteins and eosinophil survival activity (r(s) = 0.717 to 0.880, p < 0.001) in BAL fluids. Eosinophil survival activity was completely neutralized by anti-IL-5 monoclonal antibody in five of the seven 48-h samples tested representing three of the 4 patients. In the two remaining samples, eosinophil survival activity was only partially neutralized by either anti-IL-5 antibody or anti-granulocyte-macrophage colony-stimulating factor (GM-CSF) but was completely neutralized by anti-IL-5 and anti-GM-CSF in combination. Subsequently, in the second study, 10 patients with allergic rhinitis were challenged by SBP with ragweed extract. Eosinophil survival activity was significantly elevated in the 48-h BAL fluids; this activity was partially neutralized by anti-IL-5 antibody about (48{\%}) and completely neutralized by the combination of anti-IL-5 and anti-GM-CSF antibodies. These findings suggest that the eosinophil survival activity in the late inflammatory lesions following SBP with allergen is mainly associated with IL-5, with small contributions from GM-CSF. Thus, IL-5 is the predominant eosinophil-active cytokine present in BAL fluids during allergen-induced late-phase inflammation and may play a key role in the pathophysiology of allergen-induced, eosinophil-predominant airway inflammation.",
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AU - Ohnishi, T.

AU - Kita, H.

AU - Weiler, D.

AU - Sur, Sanjiv

AU - Sedgwick, J. B.

AU - Calhoun, William

AU - Busse, W. W.

AU - Abrams, J. S.

AU - Gleich, G. J.

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