TY - JOUR
T1 - Imaging alternative splicing in living cells.
AU - Wagner, Eric J.
AU - Baines, Andrea
AU - Albrecht, Todd
AU - Brazas, Robert M.
AU - Garcia-Blanco, Mariano A.
PY - 2004
Y1 - 2004
N2 - We have developed an in vivo reporter of alternative splicing decisions that allows for the determination of FGF-R2 splicing patterns without the destruction of cells. This method has broad applications, including the study of other alternatively spliced genes in tissue culture and in whole animals, and may be useful in creating imaging markers for the study of tumor progression and metastasis. In this chapter, the authors present one example of this method using fluorescence reporters. As with any new assay, a series of experiments were performed to validate the method. This chapter documents some of these experiments.
AB - We have developed an in vivo reporter of alternative splicing decisions that allows for the determination of FGF-R2 splicing patterns without the destruction of cells. This method has broad applications, including the study of other alternatively spliced genes in tissue culture and in whole animals, and may be useful in creating imaging markers for the study of tumor progression and metastasis. In this chapter, the authors present one example of this method using fluorescence reporters. As with any new assay, a series of experiments were performed to validate the method. This chapter documents some of these experiments.
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M3 - Article
C2 - 14769994
AN - SCOPUS:2142820823
SN - 1064-3745
VL - 257
SP - 29
EP - 46
JO - Methods in molecular biology (Clifton, N.J.)
JF - Methods in molecular biology (Clifton, N.J.)
ER -