Imaging alternative splicing in living cells.

Eric J. Wagner, Andrea Baines, Todd Albrecht, Robert M. Brazas, Mariano A. Garcia-Blanco

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

We have developed an in vivo reporter of alternative splicing decisions that allows for the determination of FGF-R2 splicing patterns without the destruction of cells. This method has broad applications, including the study of other alternatively spliced genes in tissue culture and in whole animals, and may be useful in creating imaging markers for the study of tumor progression and metastasis. In this chapter, the authors present one example of this method using fluorescence reporters. As with any new assay, a series of experiments were performed to validate the method. This chapter documents some of these experiments.

Original languageEnglish (US)
Pages (from-to)29-46
Number of pages18
JournalMethods in molecular biology (Clifton, N.J.)
Volume257
StatePublished - 2004
Externally publishedYes

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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