Imaging alternative splicing in living cells.

Eric Wagner, Andrea Baines, Todd Albrecht, Robert M. Brazas, Mariano Garcia-Blanco

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

We have developed an in vivo reporter of alternative splicing decisions that allows for the determination of FGF-R2 splicing patterns without the destruction of cells. This method has broad applications, including the study of other alternatively spliced genes in tissue culture and in whole animals, and may be useful in creating imaging markers for the study of tumor progression and metastasis. In this chapter, the authors present one example of this method using fluorescence reporters. As with any new assay, a series of experiments were performed to validate the method. This chapter documents some of these experiments.

Original languageEnglish (US)
Pages (from-to)29-46
Number of pages18
JournalMethods in molecular biology (Clifton, N.J.)
Volume257
StatePublished - 2004
Externally publishedYes

Fingerprint

Alternative Splicing
Recombinant DNA
Tumor Biomarkers
Fluorescence
Neoplasm Metastasis

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Imaging alternative splicing in living cells. / Wagner, Eric; Baines, Andrea; Albrecht, Todd; Brazas, Robert M.; Garcia-Blanco, Mariano.

In: Methods in molecular biology (Clifton, N.J.), Vol. 257, 2004, p. 29-46.

Research output: Contribution to journalArticle

Wagner, Eric ; Baines, Andrea ; Albrecht, Todd ; Brazas, Robert M. ; Garcia-Blanco, Mariano. / Imaging alternative splicing in living cells. In: Methods in molecular biology (Clifton, N.J.). 2004 ; Vol. 257. pp. 29-46.
@article{b02814e1343e43f69ad9f2b0dfd08271,
title = "Imaging alternative splicing in living cells.",
abstract = "We have developed an in vivo reporter of alternative splicing decisions that allows for the determination of FGF-R2 splicing patterns without the destruction of cells. This method has broad applications, including the study of other alternatively spliced genes in tissue culture and in whole animals, and may be useful in creating imaging markers for the study of tumor progression and metastasis. In this chapter, the authors present one example of this method using fluorescence reporters. As with any new assay, a series of experiments were performed to validate the method. This chapter documents some of these experiments.",
author = "Eric Wagner and Andrea Baines and Todd Albrecht and Brazas, {Robert M.} and Mariano Garcia-Blanco",
year = "2004",
language = "English (US)",
volume = "257",
pages = "29--46",
journal = "Methods in Molecular Biology",
issn = "1064-3745",
publisher = "Humana Press",

}

TY - JOUR

T1 - Imaging alternative splicing in living cells.

AU - Wagner, Eric

AU - Baines, Andrea

AU - Albrecht, Todd

AU - Brazas, Robert M.

AU - Garcia-Blanco, Mariano

PY - 2004

Y1 - 2004

N2 - We have developed an in vivo reporter of alternative splicing decisions that allows for the determination of FGF-R2 splicing patterns without the destruction of cells. This method has broad applications, including the study of other alternatively spliced genes in tissue culture and in whole animals, and may be useful in creating imaging markers for the study of tumor progression and metastasis. In this chapter, the authors present one example of this method using fluorescence reporters. As with any new assay, a series of experiments were performed to validate the method. This chapter documents some of these experiments.

AB - We have developed an in vivo reporter of alternative splicing decisions that allows for the determination of FGF-R2 splicing patterns without the destruction of cells. This method has broad applications, including the study of other alternatively spliced genes in tissue culture and in whole animals, and may be useful in creating imaging markers for the study of tumor progression and metastasis. In this chapter, the authors present one example of this method using fluorescence reporters. As with any new assay, a series of experiments were performed to validate the method. This chapter documents some of these experiments.

UR - http://www.scopus.com/inward/record.url?scp=2142820823&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=2142820823&partnerID=8YFLogxK

M3 - Article

VL - 257

SP - 29

EP - 46

JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

SN - 1064-3745

ER -