Immune protection against HSV-2 in B-cell-deficient mice

Kristen L. Dudley, Nigel Bourne, Gregg Milligan

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

The role of antibody in protection of the vaginal mucosa and sensory ganglia against HSV-2 infection was examined using HSV- immune, B-cell- deficient μMT mice. Significantly higher virus titers were detected in the vaginal mucosae of immune μMT mice compared to immune C57BL/6J mice 24 h after HSV-2 rechallenge. However, virus was rapidly cleared in immune μMT mice, and the infection was resolved with only a 2-day delay. Passive transfer of immune serum to immune μMT mice prior to rechallenge resulted in HSV-specific vaginal IgG levels comparable to those of immune C57BL/6J mice. Although transferred antibody failed to prevent reinfection of the majority of recipients, vaginal virus titers at 24 h and clearance kinetics were similar to those of immune C57BL/6J controls. Following vaginal rechallenge, HSV-2 did not spread to the sensory ganglia of immune C57BL/6J mice nor was the rechallenge virus detected in the ganglia of the majority of immune μMT mice. However, protection was severely compromised by T-cell depletion of immune C57BL/6J mice. These results suggest that HSV-specific antibody limits, but does not prevent, infection of the genital epithelia. Further, prevention of virus spread to the sensory ganglia in immune animals requires vigorous T-cell immune responses. (C) 2000 Academic Press.

Original languageEnglish (US)
Pages (from-to)454-463
Number of pages10
JournalVirology
Volume270
Issue number2
DOIs
StatePublished - May 10 2000
Externally publishedYes

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Human Herpesvirus 2
Sensory Ganglia
B-Lymphocytes
Inbred C57BL Mouse
Viruses
Viral Load
Antibodies
Mucous Membrane
Infection
T-Lymphocytes
Ganglia
Immune Sera
Epithelium
Immunoglobulin G

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Immune protection against HSV-2 in B-cell-deficient mice. / Dudley, Kristen L.; Bourne, Nigel; Milligan, Gregg.

In: Virology, Vol. 270, No. 2, 10.05.2000, p. 454-463.

Research output: Contribution to journalArticle

Dudley, Kristen L. ; Bourne, Nigel ; Milligan, Gregg. / Immune protection against HSV-2 in B-cell-deficient mice. In: Virology. 2000 ; Vol. 270, No. 2. pp. 454-463.
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