Objective: To examine CD177 expression in peripheral blood and inflamed mucosa from active patients with inflammatory bowel disease (IBD), and explore its potential role in regulating the migration of neutrophils. Methods: Peripheral blood and inflamed intestinal mucosa were harvested from patients with active Crohn′s disease (n=18), active ulcerative colitis (n=21) and healthy controls (n=14). CD177 expression was determined by flow cytometry. CD177+ and CD177- neutrophils were also isolated from peripheral blood of patients with active IBD by immunomagnetic beads, and the migration of these neutrophils was analyzed by Transwell. Results: CD177 expression was highly increased in peripheral blood neutrophils and inflamed mucosa from patients with active IBD compared with that in healthy controls (P<0.05). Purified CD177+ and CD177- neutrophils were isolated from active IBD patients, and a Transwell analysis revealed that the migration capacity of CD177+ neutrophils was markedly increased than that of CD177- neutrophils in all groups (P<0.05). Moreover, the migration capacity of CD177+ neutrophils from IBD patients was also markedly increased compared with that from healthy controls (P<0.05). Conclusion: Our data demonstrate that CD177 expression is increased in patients with IBD, and that CD177 promotes neutrophil migration and homing into intestinal mucosa. CD177+ neutrophils may play an important regulatory role in modulating intestinal mucosal inflammation.
|Translated title of the contribution||Immune regulatory mechanisms of CD177+ neutrophils in the pathogenesis of inflammatory bowel disease|
|Original language||Chinese (Traditional)|
|Number of pages||4|
|Journal||Chinese Journal of Inflammatory Bowel Diseases|
|State||Published - Apr 25 2017|
- Inflammatory bowel disease
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