Immune responses to coiled coil supramolecular biomaterials

Jai S. Rudra, Pulak K. Tripathi, David A. Hildeman, Jangwook P. Jung, Joel H. Collier

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

Self-assembly has been increasingly utilized in recent years to create peptide-based biomaterials for 3D cell culture, tissue engineering, and regenerative medicine, but the molecular determinants of these materials' immunogenicity have remained largely unexplored. In this study, a set of molecules that self-assembled through coiled coil oligomerization was designed and synthesized, and immune responses against them were investigated in mice. Experimental groups spanned a range of oligomerization behaviors and included a peptide from the coiled coil region of mouse fibrin that did not form supramolecular structures, an engineered version of this peptide that formed coiled coil bundles, and a peptide-PEG-peptide triblock bioconjugate that formed coiled coil multimers and supramolecular aggregates. In mice, the native peptide and engineered peptide did not produce any detectable antibody response, and none of the materials elicited detectable peptide-specific T cell responses, as evidenced by the absence of IL-2 and interferon-gamma in cultures of peptide-challenged splenocytes or draining lymph node cells. However, specific antibody responses were elevated in mice injected with the multimerizing peptide-PEG-peptide. Minimal changes in secondary structure were observed between the engineered peptide and the triblock peptide-PEG-peptide, making it possible that the triblock's multimerization was responsible for this antibody response.

Original languageEnglish (US)
Pages (from-to)8475-8483
Number of pages9
JournalBiomaterials
Volume31
Issue number32
DOIs
StatePublished - Nov 2010
Externally publishedYes

Fingerprint

Biocompatible Materials
Biomaterials
Peptides
Antibodies
Antibody Formation
Polyethylene glycols
Oligomerization
Peptide T
Interferons
Regenerative Medicine
Tissue culture
T-cells
Tissue Engineering
Fibrin
Tissue engineering
Interferon-gamma
Interleukin-2
Self assembly
Cell Culture Techniques
Lymph Nodes

Keywords

  • Immunogenicity
  • Regenerative medicine
  • Self-assembly
  • Tissue engineering

ASJC Scopus subject areas

  • Biomaterials
  • Bioengineering
  • Ceramics and Composites
  • Mechanics of Materials
  • Biophysics

Cite this

Rudra, J. S., Tripathi, P. K., Hildeman, D. A., Jung, J. P., & Collier, J. H. (2010). Immune responses to coiled coil supramolecular biomaterials. Biomaterials, 31(32), 8475-8483. https://doi.org/10.1016/j.biomaterials.2010.07.068

Immune responses to coiled coil supramolecular biomaterials. / Rudra, Jai S.; Tripathi, Pulak K.; Hildeman, David A.; Jung, Jangwook P.; Collier, Joel H.

In: Biomaterials, Vol. 31, No. 32, 11.2010, p. 8475-8483.

Research output: Contribution to journalArticle

Rudra, JS, Tripathi, PK, Hildeman, DA, Jung, JP & Collier, JH 2010, 'Immune responses to coiled coil supramolecular biomaterials', Biomaterials, vol. 31, no. 32, pp. 8475-8483. https://doi.org/10.1016/j.biomaterials.2010.07.068
Rudra JS, Tripathi PK, Hildeman DA, Jung JP, Collier JH. Immune responses to coiled coil supramolecular biomaterials. Biomaterials. 2010 Nov;31(32):8475-8483. https://doi.org/10.1016/j.biomaterials.2010.07.068
Rudra, Jai S. ; Tripathi, Pulak K. ; Hildeman, David A. ; Jung, Jangwook P. ; Collier, Joel H. / Immune responses to coiled coil supramolecular biomaterials. In: Biomaterials. 2010 ; Vol. 31, No. 32. pp. 8475-8483.
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