Immunization of mice against west nile virus with recombinant envelope protein

T. Wang, J. F. Anderson, L. A. Magnarelli, S. J. Wong, R. A. Koski, E. Fikrig

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Abstract

West Nile (WN) virus is a mosquito-borne flavivirus that emerged in the United States in 1999 and can cause fatal encephalitis. Envelope (E) protein cDNA from a WN virus isolate recovered from Culex pipiens in Connecticut was expressed in Escherichia coli. The recombinant E protein was purified and used as Ag in immunoblot assays and immunization experiments. Patients with WN virus infection had Abs that recognized the recombinant E protein. C3H/HeN mice immunized with E protein developed E protein Abs and were protected from infection with WN virus. Passive administration of E protein antisera was also sufficient to afford immunity. E protein is a candidate vaccine to prevent WN virus infection.

Original languageEnglish (US)
Pages (from-to)5273-5277
Number of pages5
JournalJournal of Immunology
Volume167
Issue number9
DOIs
StatePublished - Nov 1 2001
Externally publishedYes

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ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Wang, T., Anderson, J. F., Magnarelli, L. A., Wong, S. J., Koski, R. A., & Fikrig, E. (2001). Immunization of mice against west nile virus with recombinant envelope protein. Journal of Immunology, 167(9), 5273-5277. https://doi.org/10.4049/jimmunol.167.9.5273