Immunization of mice against west nile virus with recombinant envelope protein

Tian Wang, J. F. Anderson, L. A. Magnarelli, S. J. Wong, R. A. Koski, E. Fikrig

Research output: Contribution to journalArticle

82 Citations (Scopus)

Abstract

West Nile (WN) virus is a mosquito-borne flavivirus that emerged in the United States in 1999 and can cause fatal encephalitis. Envelope (E) protein cDNA from a WN virus isolate recovered from Culex pipiens in Connecticut was expressed in Escherichia coli. The recombinant E protein was purified and used as Ag in immunoblot assays and immunization experiments. Patients with WN virus infection had Abs that recognized the recombinant E protein. C3H/HeN mice immunized with E protein developed E protein Abs and were protected from infection with WN virus. Passive administration of E protein antisera was also sufficient to afford immunity. E protein is a candidate vaccine to prevent WN virus infection.

Original languageEnglish (US)
Pages (from-to)5273-5277
Number of pages5
JournalJournal of Immunology
Volume167
Issue number9
StatePublished - Nov 1 2001
Externally publishedYes

Fingerprint

West Nile virus
Recombinant Proteins
Immunization
Virus Diseases
Proteins
Flavivirus
Culex
Inbred C3H Mouse
Encephalitis
Culicidae
Immune Sera
Immunity
Vaccines
Complementary DNA
Escherichia coli
Infection

ASJC Scopus subject areas

  • Immunology

Cite this

Wang, T., Anderson, J. F., Magnarelli, L. A., Wong, S. J., Koski, R. A., & Fikrig, E. (2001). Immunization of mice against west nile virus with recombinant envelope protein. Journal of Immunology, 167(9), 5273-5277.

Immunization of mice against west nile virus with recombinant envelope protein. / Wang, Tian; Anderson, J. F.; Magnarelli, L. A.; Wong, S. J.; Koski, R. A.; Fikrig, E.

In: Journal of Immunology, Vol. 167, No. 9, 01.11.2001, p. 5273-5277.

Research output: Contribution to journalArticle

Wang, T, Anderson, JF, Magnarelli, LA, Wong, SJ, Koski, RA & Fikrig, E 2001, 'Immunization of mice against west nile virus with recombinant envelope protein', Journal of Immunology, vol. 167, no. 9, pp. 5273-5277.
Wang T, Anderson JF, Magnarelli LA, Wong SJ, Koski RA, Fikrig E. Immunization of mice against west nile virus with recombinant envelope protein. Journal of Immunology. 2001 Nov 1;167(9):5273-5277.
Wang, Tian ; Anderson, J. F. ; Magnarelli, L. A. ; Wong, S. J. ; Koski, R. A. ; Fikrig, E. / Immunization of mice against west nile virus with recombinant envelope protein. In: Journal of Immunology. 2001 ; Vol. 167, No. 9. pp. 5273-5277.
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