Immunization of mice with T cell-dependent antigens promotes IL-6 and TNF-α production in muscle cells

Erdem Tüzün, Jing Li, Nanchaya Wanasen, Lynn Soong, Premkumar Christadoss

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

IL-6 and TNF-α are proinflammatory cytokines involved in various inflammatory or non-inflammatory disorders characterized by muscle wasting. While infiltrating leukocytes are known to be the major source of these cytokines, it is unclear whether muscle cells also contribute to local inflammation. In this study, we first showed that cultured muscle cells and naive mouse muscle tissue were capable of producing IL-6 and TNF-α. We demonstrated an increased expression of IL-6 and TNF-α on muscle sections of C57BL/6J mice immunized with acetylcholine receptor (AChR) in the complete Freund's adjuvant (CFA) or with CFA only. In the presence of IL-6 or TNF-α, cultured AChR-expressing mouse (G-8) and human (TE671) skeletal muscle cells showed significantly decreased α-bungarotoxin-binding sites as measured by cellular ELISA. Moreover, IL-6- or TNF-α-treated muscle cells displayed a considerable increase in apoptotic cell ratios. Collectively, this study suggests a direct role for these two cytokines in muscle cell destruction and a possibility of muscle cell damage via an autocrine fashion.

Original languageEnglish (US)
Pages (from-to)100-106
Number of pages7
JournalCytokine
Volume35
Issue number1-2
DOIs
StatePublished - Jul 1 2006

Keywords

  • Acetylcholine receptor
  • Autoimmunity
  • IL-6
  • Myasthenia gravis
  • TNF-α

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Biochemistry
  • Hematology
  • Molecular Biology

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