Immunization with a portion of rickettsial outer membrane protein A stimulates protective immunity against spotted fever rickettsiosis

P. A. Crocquet-Valdes; C. M. Díaz-Montero; H. M. Feng; H. Li; A. D.T. Barrett; D. H. Walker

doi:10.1016/S0264-410X(01)00377-2

Immunization with a portion of rickettsial outer membrane protein A stimulates protective immunity against spotted fever rickettsiosis

P. A. Crocquet-Valdes, C. M. Díaz-Montero, H. M. Feng, H. Li, A. D.T. Barrett, D. H. Walker

Pathology

Research output: Contribution to journal › Article › peer-review

64 Scopus citations

Abstract

Two approaches for presentation of a part of the rickettsial outer membrane protein A (OmpA) of Rickettsia rickettsii, namely (1) recombinant Mycobacterium vaccae (rMV) or (2) recombinant DNA vaccine, stimulated protective immunity against a lethal challenge with the closely related bacterium, R. conorii, in mice. After primary immunization with rMV and booster immunization with homologous recombinant protein, 67 and 55% of mice were protected against challenge in two experiments. DNA vaccination with booster recombinant protein immunization protected six out of eight animals from a lethal challenge. Production of IFN-γ by antigen-exposed T-lymphocytes of DNA vaccine recipients indicated that cellular immunity had been stimulated.

Original language	English (US)
Pages (from-to)	979-988
Number of pages	10
Journal	Vaccine
Volume	20
Issue number	5-6
DOIs	https://doi.org/10.1016/S0264-410X(01)00377-2
State	Published - Dec 12 2001

Keywords

OmpA
Recombinant Mycobacterium vaccae
Rickettsia conorii

ASJC Scopus subject areas

Molecular Medicine
General Immunology and Microbiology
General Veterinary
Public Health, Environmental and Occupational Health
Infectious Diseases

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10.1016/S0264-410X(01)00377-2

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title = "Immunization with a portion of rickettsial outer membrane protein A stimulates protective immunity against spotted fever rickettsiosis",

abstract = "Two approaches for presentation of a part of the rickettsial outer membrane protein A (OmpA) of Rickettsia rickettsii, namely (1) recombinant Mycobacterium vaccae (rMV) or (2) recombinant DNA vaccine, stimulated protective immunity against a lethal challenge with the closely related bacterium, R. conorii, in mice. After primary immunization with rMV and booster immunization with homologous recombinant protein, 67 and 55% of mice were protected against challenge in two experiments. DNA vaccination with booster recombinant protein immunization protected six out of eight animals from a lethal challenge. Production of IFN-γ by antigen-exposed T-lymphocytes of DNA vaccine recipients indicated that cellular immunity had been stimulated.",

keywords = "OmpA, Recombinant Mycobacterium vaccae, Rickettsia conorii",

author = "Crocquet-Valdes, {P. A.} and D{\'i}az-Montero, {C. M.} and Feng, {H. M.} and H. Li and Barrett, {A. D.T.} and Walker, {D. H.}",

note = "Funding Information: We thank Haolin Ni and David Niesel for helpful discussions and suggestions and Chao Hong for his technical assistance during immunizations. This research was supported by a grant from the National Institute of Allergy and Infectious Diseases (AI 21242) and funded in part by a grant from the State of Texas Advanced Technology Program. Marcela D{\'ı}az-Montero is the recipient of a pre-doctoral fellowship from the Clayton fund. ",

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doi = "10.1016/S0264-410X(01)00377-2",

language = "English (US)",

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AU - Crocquet-Valdes, P. A.

AU - Díaz-Montero, C. M.

AU - Feng, H. M.

AU - Li, H.

AU - Barrett, A. D.T.

AU - Walker, D. H.

N1 - Funding Information: We thank Haolin Ni and David Niesel for helpful discussions and suggestions and Chao Hong for his technical assistance during immunizations. This research was supported by a grant from the National Institute of Allergy and Infectious Diseases (AI 21242) and funded in part by a grant from the State of Texas Advanced Technology Program. Marcela Dı́az-Montero is the recipient of a pre-doctoral fellowship from the Clayton fund.

PY - 2001/12/12

Y1 - 2001/12/12

N2 - Two approaches for presentation of a part of the rickettsial outer membrane protein A (OmpA) of Rickettsia rickettsii, namely (1) recombinant Mycobacterium vaccae (rMV) or (2) recombinant DNA vaccine, stimulated protective immunity against a lethal challenge with the closely related bacterium, R. conorii, in mice. After primary immunization with rMV and booster immunization with homologous recombinant protein, 67 and 55% of mice were protected against challenge in two experiments. DNA vaccination with booster recombinant protein immunization protected six out of eight animals from a lethal challenge. Production of IFN-γ by antigen-exposed T-lymphocytes of DNA vaccine recipients indicated that cellular immunity had been stimulated.

AB - Two approaches for presentation of a part of the rickettsial outer membrane protein A (OmpA) of Rickettsia rickettsii, namely (1) recombinant Mycobacterium vaccae (rMV) or (2) recombinant DNA vaccine, stimulated protective immunity against a lethal challenge with the closely related bacterium, R. conorii, in mice. After primary immunization with rMV and booster immunization with homologous recombinant protein, 67 and 55% of mice were protected against challenge in two experiments. DNA vaccination with booster recombinant protein immunization protected six out of eight animals from a lethal challenge. Production of IFN-γ by antigen-exposed T-lymphocytes of DNA vaccine recipients indicated that cellular immunity had been stimulated.

KW - OmpA

KW - Recombinant Mycobacterium vaccae

KW - Rickettsia conorii

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Immunization with a portion of rickettsial outer membrane protein A stimulates protective immunity against spotted fever rickettsiosis

Abstract

Keywords

ASJC Scopus subject areas

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