Immunization with a portion of rickettsial outer membrane protein A stimulates protective immunity against spotted fever rickettsiosis

P. A. Crocquet-Valdes, C. M. Díaz-Montero, H. M. Feng, H. Li, Alan Barrett, David Walker

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

Two approaches for presentation of a part of the rickettsial outer membrane protein A (OmpA) of Rickettsia rickettsii, namely (1) recombinant Mycobacterium vaccae (rMV) or (2) recombinant DNA vaccine, stimulated protective immunity against a lethal challenge with the closely related bacterium, R. conorii, in mice. After primary immunization with rMV and booster immunization with homologous recombinant protein, 67 and 55% of mice were protected against challenge in two experiments. DNA vaccination with booster recombinant protein immunization protected six out of eight animals from a lethal challenge. Production of IFN-γ by antigen-exposed T-lymphocytes of DNA vaccine recipients indicated that cellular immunity had been stimulated.

Original languageEnglish (US)
Pages (from-to)979-988
Number of pages10
JournalVaccine
Volume20
Issue number5-6
DOIs
StatePublished - Dec 12 2001

Fingerprint

Mycobacterium vaccae
DNA Vaccines
outer membrane proteins
recombinant vaccines
Mycobacterium
lethal genes
Recombinant Proteins
recombinant proteins
fever
Immunity
Immunization
immunization
Fever
Rickettsia rickettsii
immunity
Secondary Immunization
mice
Cellular Immunity
cell-mediated immunity
Vaccination

Keywords

  • OmpA
  • Recombinant Mycobacterium vaccae
  • Rickettsia conorii

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Virology
  • Infectious Diseases
  • Public Health, Environmental and Occupational Health
  • veterinary(all)

Cite this

Immunization with a portion of rickettsial outer membrane protein A stimulates protective immunity against spotted fever rickettsiosis. / Crocquet-Valdes, P. A.; Díaz-Montero, C. M.; Feng, H. M.; Li, H.; Barrett, Alan; Walker, David.

In: Vaccine, Vol. 20, No. 5-6, 12.12.2001, p. 979-988.

Research output: Contribution to journalArticle

Crocquet-Valdes, P. A. ; Díaz-Montero, C. M. ; Feng, H. M. ; Li, H. ; Barrett, Alan ; Walker, David. / Immunization with a portion of rickettsial outer membrane protein A stimulates protective immunity against spotted fever rickettsiosis. In: Vaccine. 2001 ; Vol. 20, No. 5-6. pp. 979-988.
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