Immunization with a portion of rickettsial outer membrane protein A stimulates protective immunity against spotted fever rickettsiosis

P. A. Crocquet-Valdes, C. M. Díaz-Montero, H. M. Feng, H. Li, A. D.T. Barrett, D. H. Walker

Research output: Contribution to journalArticle

53 Scopus citations

Abstract

Two approaches for presentation of a part of the rickettsial outer membrane protein A (OmpA) of Rickettsia rickettsii, namely (1) recombinant Mycobacterium vaccae (rMV) or (2) recombinant DNA vaccine, stimulated protective immunity against a lethal challenge with the closely related bacterium, R. conorii, in mice. After primary immunization with rMV and booster immunization with homologous recombinant protein, 67 and 55% of mice were protected against challenge in two experiments. DNA vaccination with booster recombinant protein immunization protected six out of eight animals from a lethal challenge. Production of IFN-γ by antigen-exposed T-lymphocytes of DNA vaccine recipients indicated that cellular immunity had been stimulated.

Original languageEnglish (US)
Pages (from-to)979-988
Number of pages10
JournalVaccine
Volume20
Issue number5-6
DOIs
StatePublished - Dec 12 2001

Keywords

  • OmpA
  • Recombinant Mycobacterium vaccae
  • Rickettsia conorii

ASJC Scopus subject areas

  • Molecular Medicine
  • Immunology and Microbiology(all)
  • veterinary(all)
  • Public Health, Environmental and Occupational Health
  • Infectious Diseases

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