Immunization with fibrillar Aβ1-42 in young and aged canines: Antibody generation and characteristics, and effects on CSF and brain Aβ

Elizabeth Head, Edward G. Barrett, M. Paul Murphy, Pritam Das, Mihaela Nistor, Floyd Sarsoza, Charles C. Glabe, Rakez Kayed, Saskia Milton, Vitaly Vasilevko, Norton W. Milgram, Michael G. Agadjanyan, David H. Cribbs, Carl W. Cotman

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

We describe a study testing fibrillar β-amyloid1-42 (Aβ42) vaccination in dogs. Three young beagles (4.6 years) were immunized twice with Aβ42 and a Th1 adjuvant (TiterMax Gold). Animals generated primarily IgG2 and IgM antibody responses, which were specific for the Aβ11-30 region of Aβ1-42. Next, 3 aged beagles (8.9-13.8 years) were immunized 4 times with Aβ42 and a Th2 adjuvant (Alum). We observed an acute increase in IgG2, a slower increase in IgG1 and Aβ antibodies of broader specificity (Aβ1-15>11-30>6-20). A nonsignificant increase in CSF Aβ1-40 and decrease in Aβ1-40/1-42 in cortex was detected. Canines may be a useful system for testing an Aβ vaccine.

Original languageEnglish (US)
Pages (from-to)2824-2834
Number of pages11
JournalVaccine
Volume24
Issue number15
DOIs
StatePublished - Apr 5 2006
Externally publishedYes

Keywords

  • Alum
  • Alzheimer's disease
  • Beta-amyloid
  • Brain aging
  • Dog

ASJC Scopus subject areas

  • Molecular Medicine
  • Immunology and Microbiology(all)
  • veterinary(all)
  • Public Health, Environmental and Occupational Health
  • Infectious Diseases

Fingerprint Dive into the research topics of 'Immunization with fibrillar Aβ<sub>1-42</sub> in young and aged canines: Antibody generation and characteristics, and effects on CSF and brain Aβ'. Together they form a unique fingerprint.

  • Cite this

    Head, E., Barrett, E. G., Murphy, M. P., Das, P., Nistor, M., Sarsoza, F., Glabe, C. C., Kayed, R., Milton, S., Vasilevko, V., Milgram, N. W., Agadjanyan, M. G., Cribbs, D. H., & Cotman, C. W. (2006). Immunization with fibrillar Aβ1-42 in young and aged canines: Antibody generation and characteristics, and effects on CSF and brain Aβ. Vaccine, 24(15), 2824-2834. https://doi.org/10.1016/j.vaccine.2005.12.058