Immunoblot studies of the IGF-related acid-labile subunit

F. Liu, R. L. Hintz, A. Khare, R. P. Diaugustine, D. R. Powell, Phillip Lee

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Insulin-like growth factors I and II (IGF-I and II) are present in serum primarily within a ternary complex consisting of IGF, IGF-binding protein- 3 (IGF-3) and acid-labile subunit (ALS). Relatively little is known about ALS as compared to the other components of the complex. We report immunoblot studies of ALS using a new rabbit antiserum to human ALS1-34. The antiserum shows high specificity for ALS, labelling only the intact 82-88 kDa doublet in whole serum. Treatment with endoglycosidase-F leads to only a partial deglycosylation of ALS in whole serum, while purified ALS is reduced to Mr~58 kDa. Acidification of both whole serum and purified ALS leads to a complete loss of ALS ability to bind to cross-linked IGFBP- 3:[125I]IGF-II tracer; however, immunoblot studies show no change in the apparent Mr of the major ALS band. Immunoblot studies of human serum shows that intact ALS is decreased in growth-hormone (GH) deficiency, increases with GH treatment, is elevated in GH excess and is unchanged with IGF-I treatment. These data provide new information regarding the characteristics of ALS and demonstrate the research utility of a highly-specific antiserum for this protein.

Original languageEnglish (US)
Pages (from-to)1883-1886
Number of pages4
JournalJournal of Clinical Endocrinology and Metabolism
Volume79
Issue number6
StatePublished - 1994
Externally publishedYes

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Acids
Insulin-Like Growth Factor II
Insulin-Like Growth Factor I
Growth Hormone
Immune Sera
Serum
Insulin-Like Growth Factor Binding Protein 3
insulin-like growth factor binding protein, acid labile subunit
Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase
Acidification
Labeling
Rabbits
Research
Proteins

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology, Diabetes and Metabolism

Cite this

Liu, F., Hintz, R. L., Khare, A., Diaugustine, R. P., Powell, D. R., & Lee, P. (1994). Immunoblot studies of the IGF-related acid-labile subunit. Journal of Clinical Endocrinology and Metabolism, 79(6), 1883-1886.

Immunoblot studies of the IGF-related acid-labile subunit. / Liu, F.; Hintz, R. L.; Khare, A.; Diaugustine, R. P.; Powell, D. R.; Lee, Phillip.

In: Journal of Clinical Endocrinology and Metabolism, Vol. 79, No. 6, 1994, p. 1883-1886.

Research output: Contribution to journalArticle

Liu, F, Hintz, RL, Khare, A, Diaugustine, RP, Powell, DR & Lee, P 1994, 'Immunoblot studies of the IGF-related acid-labile subunit', Journal of Clinical Endocrinology and Metabolism, vol. 79, no. 6, pp. 1883-1886.
Liu F, Hintz RL, Khare A, Diaugustine RP, Powell DR, Lee P. Immunoblot studies of the IGF-related acid-labile subunit. Journal of Clinical Endocrinology and Metabolism. 1994;79(6):1883-1886.
Liu, F. ; Hintz, R. L. ; Khare, A. ; Diaugustine, R. P. ; Powell, D. R. ; Lee, Phillip. / Immunoblot studies of the IGF-related acid-labile subunit. In: Journal of Clinical Endocrinology and Metabolism. 1994 ; Vol. 79, No. 6. pp. 1883-1886.
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