Immunocytes and abnormal gastrointestinal motor activity during ileitis in dogs

P. Jouet, S. K. Sarna, C. Singaram, R. P. Ryan, C. J. Hillard, G. L. Telford, J. Fink, J. D. Henderson

Research output: Contribution to journalArticle

35 Scopus citations

Abstract

Infiltration of specific immunocytes and stimulation of abnormal gastrointestinal motor activity during ileal inflammation induced by mucosal exposure to ethanol and acetic acid were investigated in 17 dogs. Ileal inflammation significantly increased the frequency of giant migrating contractions (GMCs) and decreased the frequency of migrating motor complexes (MMCs). The frequency of retrograde giant contractions (RGCs) increased only on the day of ethanol and acetic acid treatment. Diarrhea, urgency of defecation, and apparent abdominal discomfort were related to the increased frequency of GMCs. Ileal inflammation also prolonged the duration of postprandial MMC disruption. Histological and immunohistochemical findings indicated transmural inflammation with marked increase in polymorphonuclear cells in the lamina propria and muscularis externa layers. Myeloperoxidase activity increased severalfold in both layers. Cells containing interleukin- 2 receptor (IL-2R) increased in the lamina propria. Other immunocytes, such as B and T lymphocytes, dendritic cells, and human leukocyte antigen DR-1 (HLADR)-positive cells, did not exhibit a significant increase in the inflamed ileum compared with the normal proximal jejunum. We conclude that stimulation of GMCs may be the major motility marker of intestinal inflammation.

Original languageEnglish (US)
Pages (from-to)G913-G924
JournalAmerican Journal of Physiology - Gastrointestinal and Liver Physiology
Volume269
Issue number6 32-6
DOIs
StatePublished - Jan 1 1995

Keywords

  • Crohn's disease
  • diarrhea
  • inflammatory bowel disease
  • motility

ASJC Scopus subject areas

  • Physiology
  • Hepatology
  • Gastroenterology
  • Physiology (medical)

Fingerprint Dive into the research topics of 'Immunocytes and abnormal gastrointestinal motor activity during ileitis in dogs'. Together they form a unique fingerprint.

  • Cite this

    Jouet, P., Sarna, S. K., Singaram, C., Ryan, R. P., Hillard, C. J., Telford, G. L., Fink, J., & Henderson, J. D. (1995). Immunocytes and abnormal gastrointestinal motor activity during ileitis in dogs. American Journal of Physiology - Gastrointestinal and Liver Physiology, 269(6 32-6), G913-G924. https://doi.org/10.1152/ajpgi.1995.269.6.g913